Structural basis for the gating mechanism of the type 2 ryanodine receptor RyR2

Wei Peng, Huaizong Shen, Jianping Wu, Wenting Guo, Xiaojing Pan, Ruiwu Wang, S. R.Wayne Chen, Nieng Yan

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

RyR2 is a high-conductance intracellular calcium (Ca2+) channel that controls the release of Ca2+ from the sarco(endo)plasmic reticulum of a variety of cells. Here, we report the structures of RyR2 from porcine heart in both the open and closed states at near-atomic resolutions determined using single-particle electron cryomicroscopy. Structural comparison reveals a breathing motion of the overall cytoplasmic region resulted from the interdomain movements of amino-terminal domains (NTDs), Helical domains, and Handle domains, whereas almost no intradomain shifts are observed in these armadillo repeats-containing domains. Outward rotations of the Central domains, which integrate the conformational changes of the cytoplasmic region, lead to the dilation of the cytoplasmic gate through coupled motions. Our structural and mutational characterizations provide important insights into the gating and disease mechanism of RyRs.

Original languageEnglish (US)
Article numberaah5324
JournalScience
Volume354
Issue number6310
DOIs
StatePublished - Oct 21 2016
Externally publishedYes

All Science Journal Classification (ASJC) codes

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    Peng, W., Shen, H., Wu, J., Guo, W., Pan, X., Wang, R., Chen, S. R. W., & Yan, N. (2016). Structural basis for the gating mechanism of the type 2 ryanodine receptor RyR2. Science, 354(6310), [aah5324]. https://doi.org/10.1126/science.aah5324