Structural basis for sequence-specific recognition of DNA by TAL effectors

Dong Deng, Chuangye Yan, Xiaojing Pan, Magdy Mahfouz, Jiawei Wang, Jian Kang Zhu, Yigong Shi, Nieng Yan

Research output: Contribution to journalArticle

366 Scopus citations

Abstract

TAL (transcription activator-like) effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair by using two hypervariable residues [known as repeat variable diresidues (RVDs)] at positions 12 and 13. Here, we report the crystal structures of an 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, superhelical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications.

Original languageEnglish (US)
Pages (from-to)720-723
Number of pages4
JournalScience
Volume335
Issue number6069
DOIs
StatePublished - Feb 10 2012

All Science Journal Classification (ASJC) codes

  • General

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    Deng, D., Yan, C., Pan, X., Mahfouz, M., Wang, J., Zhu, J. K., Shi, Y., & Yan, N. (2012). Structural basis for sequence-specific recognition of DNA by TAL effectors. Science, 335(6069), 720-723. https://doi.org/10.1126/science.1215670