Abstract
The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.
Original language | English (US) |
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Pages (from-to) | 1775-1782 |
Number of pages | 8 |
Journal | Biotechnology Letters |
Volume | 31 |
Issue number | 11 |
DOIs | |
State | Published - Oct 2009 |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Keywords
- G protein-coupled receptors
- Heterologous protein expression
- Sigma factor
- Stress response