Stress responses to heterologous membrane protein expression in Escherichia coli

Lucy Y. Xu, A. James Link

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The stress response of E. coli to the expression of two recombinant membrane proteins, the E. coli AAA+ protease FtsH and the human G-protein coupled receptor CB1, was examined using several members of a promoter-GFP library. Several genes from the heat-shock and envelope stress regulons (rpoH, clpP, lon, and ftsH) were strongly induced by expression of either membrane protein. Flow cytometry was used to monitor the real-time dynamics of the transcription of these reporter genes in response to membrane protein expression. Co-expression of CB1 and FtsH led to an additive response in these four reporter genes suggesting that the stresses may be occurring via different physiological mechanisms.

Original languageEnglish (US)
Pages (from-to)1775-1782
Number of pages8
JournalBiotechnology Letters
Volume31
Issue number11
DOIs
StatePublished - Oct 1 2009

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Keywords

  • G protein-coupled receptors
  • Heterologous protein expression
  • Sigma factor
  • Stress response

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