@article{97f32aa695184a8da037965525e8841d,
title = "Stress ball morphogenesis: How the lizard builds its lung",
abstract = "The function of the lung is closely coupled to its structural anatomy, which varies greatly across vertebrates. Although architecturally simple, a complex pattern of airflow is thought to be achieved in the lizard lung due to its cavernous central lumen and honeycomb-shaped wall. We find that the wall of the lizard lung is generated from an initially smooth epithelial sheet, which is pushed through holes in a hexagonal smooth muscle meshwork by forces from fluid pressure, similar to a stress ball. Combining transcriptomics with time-lapse imaging reveals that the hexagonal meshwork self-assembles in response to circumferential and axial stresses downstream of pressure. A computational model predicts the pressure-driven changes in epithelial topology, which we probe using optogenetically driven contraction of 3D-printed engineered muscle. These results reveal the physical principles used to sculpt the unusual architecture of the lizard lung, which could be exploited as a novel strategy to engineer tissues.",
author = "Palmer, {Michael A.} and Nerger, {Bryan A.} and Katharine Goodwin and Anvitha Sudhakar and Lemke, {Sandra B.} and Ravindran, {Pavithran T.} and Toettcher, {Jared E.} and Andrej Ko{\v s}mrlj and Nelson, {Celeste M.}",
note = "Funding Information: This work was supported in part by the NIH (HL110335, HL118532, HL120142, and HD099030 to C.M.N. and EB024247 to J.E.T), the NSF (CMMI-1435853 to C.M.N. and CAREER 1750663 to J.E.T.), the Eric and Wendy Schmidt Transformative Technology Fund, and a Faculty Scholars Award from the Howard Hughes Medical Institute (to C.M.N.). B.A.N. and K.G. were supported in part by the postgraduate scholarship-doctoral (PGS-D) program of the Natural Sciences and Engineering Research Council of Canada. K.G. was supported in part by the Dr. Margaret McWilliams Predoctoral Fellowship from the Canadian Federation of University Women. We acknowledge the use of the Princeton University Imaging and Analysis Center, the PCCM Materials Research Science and Engineering Center (NSF DMR-2011750), the Genomics Core Facility, and the Molecular Biology Confocal Microscopy Facility. Publisher Copyright: Copyright {\textcopyright} 2021 The Authors, some rights reserved.",
year = "2021",
month = dec,
doi = "10.1126/sciadv.abk0161",
language = "English (US)",
volume = "7",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "52",
}