Stalled Hox promoters as chromosomal boundaries

Vivek S. Chopra, Jessica Cande, Joung Woo Hong, Michael Levine

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Many developmental control genes contain stalled RNA Polymerase II (Pol II) in the early Drosophila embryo, including four of the eight Hox genes. Here, we present evidence that the stalled Hox promoters possess an intrinsic insulator activity. The enhancer-blocking activities of these promoters are dependent on general transcription factors that inhibit Pol II elongation, including components of the DSIF and NELF complexes. The activities of conventional insulators are also impaired in embryos containing reduced levels of DSIF and NELF. Thus, promoter-proximal stalling factors might help promote insulator-promoter interactions. We propose that stalled promoters help organize gene complexes within chromosomal loop domains.

Original languageEnglish (US)
Pages (from-to)1505-1509
Number of pages5
JournalGenes and Development
Volume23
Issue number13
DOIs
StatePublished - Jul 1 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • DSIF
  • Hox
  • Insulator
  • Long range
  • NELF
  • Pol II stalling

Fingerprint Dive into the research topics of 'Stalled Hox promoters as chromosomal boundaries'. Together they form a unique fingerprint.

  • Cite this