Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Jonathan S. Marvin, Benjamin Scholl, Daniel E. Wilson, Kaspar Podgorski, Abbas Kazemipour, Johannes Alexander Müller, Susanne Schoch, Francisco José Urra Quiroz, Nelson Rebola, Huan Bao, Justin P. Little, Ariana N. Tkachuk, Edward Cai, Adam W. Hantman, Samuel S.H. Wang, Victor J. DePiero, Bart G. Borghuis, Edwin R. Chapman, Dirk Dietrich, David A. DiGregorioDavid Fitzpatrick, Loren L. Looger

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.

Original languageEnglish (US)
Pages (from-to)936-939
Number of pages4
JournalNature Methods
Volume15
Issue number11
DOIs
StatePublished - Nov 1 2018

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Marvin, J. S., Scholl, B., Wilson, D. E., Podgorski, K., Kazemipour, A., Müller, J. A., Schoch, S., Quiroz, F. J. U., Rebola, N., Bao, H., Little, J. P., Tkachuk, A. N., Cai, E., Hantman, A. W., Wang, S. S. H., DePiero, V. J., Borghuis, B. G., Chapman, E. R., Dietrich, D., ... Looger, L. L. (2018). Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nature Methods, 15(11), 936-939. https://doi.org/10.1038/s41592-018-0171-3