Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Jonathan S. Marvin; Benjamin Scholl; Daniel E. Wilson; Kaspar Podgorski; Abbas Kazemipour; Johannes Alexander Müller; Susanne Schoch; Francisco José Urra Quiroz; Nelson Rebola; Huan Bao; Justin P. Little; Ariana N. Tkachuk; Edward Cai; Adam W. Hantman; Samuel S.H. Wang; Victor J. DePiero; Bart G. Borghuis; Edwin R. Chapman; Dirk Dietrich; David A. DiGregorio; David Fitzpatrick; Loren L. Looger

doi:10.1038/s41592-018-0171-3

Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Jonathan S. Marvin, Benjamin Scholl, Daniel E. Wilson, Kaspar Podgorski, Abbas Kazemipour, Johannes Alexander Müller, Susanne Schoch, Francisco José Urra Quiroz, Nelson Rebola, Huan Bao, Justin P. Little, Ariana N. Tkachuk, Edward Cai, Adam W. Hantman, Samuel S.H. Wang, Victor J. DePiero, Bart G. Borghuis, Edwin R. Chapman, Dirk Dietrich, David A. DiGregorioDavid Fitzpatrick, Loren L. Looger

Research output: Contribution to journal › Article › peer-review

262 Scopus citations

Abstract

Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.

Original language	English (US)
Pages (from-to)	936-939
Number of pages	4
Journal	Nature Methods
Volume	15
Issue number	11
DOIs	https://doi.org/10.1038/s41592-018-0171-3
State	Published - Nov 1 2018

All Science Journal Classification (ASJC) codes

Molecular Biology
Biochemistry
Biotechnology
Cell Biology

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Marvin, J. S., Scholl, B., Wilson, D. E., Podgorski, K., Kazemipour, A., Müller, J. A., Schoch, S., Quiroz, F. J. U., Rebola, N., Bao, H., Little, J. P., Tkachuk, A. N., Cai, E., Hantman, A. W., Wang, S. S. H., DePiero, V. J., Borghuis, B. G., Chapman, E. R., Dietrich, D., ... Looger, L. L. (2018). Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR. Nature Methods, 15(11), 936-939. https://doi.org/10.1038/s41592-018-0171-3

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title = "Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR",

abstract = "Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.",

author = "Marvin, {Jonathan S.} and Benjamin Scholl and Wilson, {Daniel E.} and Kaspar Podgorski and Abbas Kazemipour and M{\"u}ller, {Johannes Alexander} and Susanne Schoch and Quiroz, {Francisco Jos{\'e} Urra} and Nelson Rebola and Huan Bao and Little, {Justin P.} and Tkachuk, {Ariana N.} and Edward Cai and Hantman, {Adam W.} and Wang, {Samuel S.H.} and DePiero, {Victor J.} and Borghuis, {Bart G.} and Chapman, {Edwin R.} and Dirk Dietrich and DiGregorio, {David A.} and David Fitzpatrick and Looger, {Loren L.}",

note = "Publisher Copyright: {\textcopyright} 2018, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2018",

month = nov,

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doi = "10.1038/s41592-018-0171-3",

language = "English (US)",

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Marvin, JS, Scholl, B, Wilson, DE, Podgorski, K, Kazemipour, A, Müller, JA, Schoch, S, Quiroz, FJU, Rebola, N, Bao, H, Little, JP, Tkachuk, AN, Cai, E, Hantman, AW, Wang, SSH, DePiero, VJ, Borghuis, BG, Chapman, ER, Dietrich, D, DiGregorio, DA, Fitzpatrick, D & Looger, LL 2018, 'Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR', Nature Methods, vol. 15, no. 11, pp. 936-939. https://doi.org/10.1038/s41592-018-0171-3

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T1 - Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

AU - Marvin, Jonathan S.

AU - Scholl, Benjamin

AU - Wilson, Daniel E.

AU - Podgorski, Kaspar

AU - Kazemipour, Abbas

AU - Müller, Johannes Alexander

AU - Schoch, Susanne

AU - Quiroz, Francisco José Urra

AU - Rebola, Nelson

AU - Bao, Huan

AU - Little, Justin P.

AU - Tkachuk, Ariana N.

AU - Cai, Edward

AU - Hantman, Adam W.

AU - Wang, Samuel S.H.

AU - DePiero, Victor J.

AU - Borghuis, Bart G.

AU - Chapman, Edwin R.

AU - Dietrich, Dirk

AU - DiGregorio, David A.

AU - Fitzpatrick, David

AU - Looger, Loren L.

PY - 2018/11/1

Y1 - 2018/11/1

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AB - Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates.

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Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

Abstract

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