spn-F encodes a novel protein that affects oocyte patterning and bristle morphology in Drosophila

Uri Abdu, Dikla Bar, Trudi Schüpbach

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The anteroposterior and dorsoventral axes of the Drosophila embryo are established during oogenesis through the activities of Gurken (Grk), a Tgfα-like protein, and the Epidermal growth factor receptor (Egfr). spn-F mutant females produce ventralized eggs similar to the phenotype produced by mutations in the grk-Egfr pathway. We found that the ventralization of the eggshell in spn-F mutants is due to defects in the localization and translation of grk mRNA during mid-oogenesis. Analysis of the microtubule network revealed defects in the organization of the microtubules around the oocyte nucleus. In addition, spn-F mutants have defective bristles. We cloned spn-F and found that it encodes a novel coiled-coil protein that localizes to the minus end of microtubules in the oocyte, and this localization requires the microtubule network and a Dynein heavy chain gene. We also show that Spn-F interacts directly with the Dynein light chain DdIc-1. Our results show that we have identified a novel protein that affects oocyte axis determination and the organization of microtubules during Drosophila oogenesis.

Original languageEnglish (US)
Pages (from-to)1477-1484
Number of pages8
JournalDevelopment
Volume133
Issue number8
DOIs
StatePublished - Apr 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Keywords

  • Bristle formation
  • Drosophila
  • Microtubule
  • Oogenesis
  • spn-F

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