Specificity of embryonic lethal mutations in Drosophila analyzed in germ line clones

Eric Wieschaus, Elizabeth Noell

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Only a small fraction of the known mutations causing death to homozygous Drosophila produce gross morphological defects during embryogenesis. We have examined fourteen such loci on the X-chromosome to determine: 1) whether the requirement for their respective activities is restricted to embryogenesis; and 2) whether the embryonic phenotype in mutant embryos is affected by the dosage of wild-type alleles in the mother. For two alleles per locus germ line clones were produced during larval development by irradiating females heterozygous for the lethal mutation and a dominant female sterile (ovoD). Only one of the 14 loci (armadillo) is required during development of the germ cell to make morphologically normal eggs. Mutations at two other loci, (bazooka and Notch), allow normal oogenesis but cause major reductions in the viability of genetically normal (i.e., heterozygous) progeny. The majority of the loci (11/14) are not required in the germ line for either oogenesis or embryogenesis. However, in three cases (extradenticle, faintoid and lethal myospheroid), germ line homozygosity results in a readily detectible enhancement of embryonic phenotype over that observed in embryos derived from heterozygous mothers still possessing one wild type allele. The same six loci which show the most substantial effects on germ line homozygosity (arm, baz, N, exd, ftd and mys) also show an amelioration of the mutant phenotypes when maternal dosage is increased to wild type levels by using attached-X females. Four of these same loci (arm, baz, N and exd were cell lethal in imaginal discs.

Original languageEnglish (US)
Pages (from-to)63-73
Number of pages11
JournalRoux's Archives of Developmental Biology
Volume195
Issue number1
DOIs
StatePublished - Jan 1 1986

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Keywords

  • Cell lethality
  • Embryonic lethals
  • Germ line clones
  • Segmentation in Drosophila

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