Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice

David W. Tank; Mutsuyuki Sugimori; John A. Connor; Rodolfo R. Llinás

doi:10.1126/science.2847315

Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice

David W. Tank, Mutsuyuki Sugimori, John A. Connor, Rodolfo R. Llinás

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297 Scopus citations

Abstract

Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.

Original language	English (US)
Pages (from-to)	773-777
Number of pages	5
Journal	Science
Volume	242
Issue number	4879
DOIs	https://doi.org/10.1126/science.2847315
State	Published - 1988
Externally published	Yes

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title = "Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice",

abstract = "Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.",

author = "Tank, {David W.} and Mutsuyuki Sugimori and Connor, {John A.} and Llin{\'a}s, {Rodolfo R.}",

year = "1988",

doi = "10.1126/science.2847315",

language = "English (US)",

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pages = "773--777",

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publisher = "American Association for the Advancement of Science",

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T1 - Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice

AU - Tank, David W.

AU - Sugimori, Mutsuyuki

AU - Connor, John A.

AU - Llinás, Rodolfo R.

PY - 1988

Y1 - 1988

N2 - Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.

AB - Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.

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JO - Science

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ER -

Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice

Abstract

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