Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice

David W. Tank, Mutsuyuki Sugimori, John A. Connor, Rodolfo R. Llinás

Research output: Contribution to journalArticle

261 Scopus citations

Abstract

Microfluorometric imaging was used to study the correlation of intracellular calcium concentration with voltage-dependent electrical activity in guinea pig cerebellar Purkinje cells. The spatiotemporal dynamics of intracellular calcium concentration are demonstrated during spontaneous and evoked activity. The results are in agreement with hypotheses of dendritic segregation of calcium conductances suggested by electrophysiological experiments. These in vitro slice fluorescence imaging methods are applicable to a wide range of problems in central nervous system biochemical and electrophysiological functions.

Original languageEnglish (US)
Pages (from-to)773-777
Number of pages5
JournalScience
Volume242
Issue number4879
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Spatially resolved calcium dynamics of mammalian Purkinje cells in cerebellar slice'. Together they form a unique fingerprint.

  • Cite this