TY - JOUR
T1 - Spatial expression of the Drosophila segment polarity gene armadillo is posttranscriptionally regulated by wingless
AU - Riggleman, Bob
AU - Schedl, Paul
AU - Wieschaus, Eric
N1 - Funding Information:
smith, Norbert Perrimon, Marcel van den Heuvel, Peter Lawrence, and Roe1 Nusse for communicating results prior to publication. We would also like to thank Edward Kennedy, Alan Frey, and Ann Fling for advice on antibody production; Dari Sweeton for preparing plastic sections of arm antibody-stained embryos; Don Doering, Shige Sakonju, and especially Mark Peifer for helpful comments on the manuscript; and our Drosophila colleagues for useful discussions during the course of this work. This research was supported by NIH Grant 5ROlHD22780 to E. W. and grants from the NIH and March of Dimes Foundation to I? S.
PY - 1990/11/2
Y1 - 1990/11/2
N2 - armadillo (arm) is one of a group of Drosophila segment polarity genes that are required for normal patterning within the embryonic segment. Although arm RNA is uniformly distributed in embryos, arm protein accumulates at higher levels in regions that contain wingless, another segment polarity gene which encodes a secreted protein that regulates patterning via cell-cell communication. These local increases in arm protein require wingless activity, and mutations that alter wingless distribution produce corresponding changes in the arm protein pattern. These results suggest that wingless regulates accumulation of arm protein by a posttranscriptional mechanism. Two other segment polarity genes, porcupine and dishevelled, are required for this effect. We also show that arm protein is closely associated with the plasma membrane in virtually all cell types and often colocalizes with F-actin.
AB - armadillo (arm) is one of a group of Drosophila segment polarity genes that are required for normal patterning within the embryonic segment. Although arm RNA is uniformly distributed in embryos, arm protein accumulates at higher levels in regions that contain wingless, another segment polarity gene which encodes a secreted protein that regulates patterning via cell-cell communication. These local increases in arm protein require wingless activity, and mutations that alter wingless distribution produce corresponding changes in the arm protein pattern. These results suggest that wingless regulates accumulation of arm protein by a posttranscriptional mechanism. Two other segment polarity genes, porcupine and dishevelled, are required for this effect. We also show that arm protein is closely associated with the plasma membrane in virtually all cell types and often colocalizes with F-actin.
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U2 - 10.1016/0092-8674(90)90451-J
DO - 10.1016/0092-8674(90)90451-J
M3 - Article
C2 - 2225066
AN - SCOPUS:0025094664
SN - 0092-8674
VL - 63
SP - 549
EP - 560
JO - Cell
JF - Cell
IS - 3
ER -