Size exclusion chromatography (SEC) of high molecular weight polymers: effects of flow rate and the creation of a universal calibration curve

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Abstract

The molecular weight (MW) and MW distribution (MWD) of water-soluble polymers was determined by size exclusion chromatography (SEC). Two series of water-soluble polymer standards (pullulan and guar) were chromatographed and a good universal calibration curve was obtained. For guar, the peak elution volume increased with flow rate by 6.5%. The flow rate effect on the MWD was also examined. Although low flow rates take longer time, they give better resolution.

Original languageEnglish (US)
Pages (from-to)1711-1712
Number of pages2
JournalAmerican Chemical Society, Polymer Preprints, Division of Polymer Chemistry
Volume41
Issue number2
StatePublished - Aug 1 2000
EventWashington,DC. Meeting - Washington, DC, USA
Duration: Aug 20 2000Aug 24 2000

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

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