Site-specific oligonucleotide binding represses transcription of the human c-myc gene in vitro

Michael Cooney, Graznya Czernuszewicz, Edith H. Postel, S. Jane Flint, Michael E. Hogan

Research output: Contribution to journalArticlepeer-review

673 Scopus citations

Abstract

A 27-base-long DNA oligonucleotide was designed that binds to duplex DNA at a single site within the 5′ end of the human c-myc gene, 115 base pairs upstream from the transcription origin P1. On the basis of the physical properties of its bound complex, it was concluded that the oligonucleotide forms a colinear triplex with the duplex binding site. By means of an in vitro assay system, it was possible to show a correlation between triplex formation at -115 base pairs and repression of c-myc transcription. The possibility is discussed that triplex formation (site-specific RNA binding to a DNA duplex) could serve as the basis for an alternative program of gene control in vivo.

Original languageEnglish (US)
Pages (from-to)456-459
Number of pages4
JournalScience
Volume241
Issue number4864
DOIs
StatePublished - Jan 1 1988

All Science Journal Classification (ASJC) codes

  • General

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