@article{e7eab7910f6145c19923e983380e313d,
title = "Single-stranded DNA and RNA origami",
abstract = "Self-folding of an information-carrying polymer into a defined structure is foundational to biology and offers attractive potential as a synthetic strategy. Although multicomponent self-assembly has produced complex synthetic nanostructures, unimolecular folding has seen limited progress.We describe a framework to design and synthesize a single DNA or RNA strand to self-fold into a complex yet unknotted structure that approximates an arbitrary user-prescribed shape. We experimentally construct diverse multikilobase single-stranded structures, including a ∼10, 000-nucleotide (nt) DNA structure and a ∼6000-nt RNA structure. We demonstrate facile replication of the strand in vitro and in living cells. The work here thus establishes unimolecular folding as a general strategy for constructing complex and replicable nucleic acid nanostructures, and expands the design space and material scalability for bottom-up nanotechnology.",
author = "Dongran Han and Xiaodong Qi and Cameron Myhrvold and Bei Wang and Mingjie Dai and Shuoxing Jiang and Maxwell Bates and Yan Liu and Byoungkwon An and Fei Zhang and Hao Yan and Peng Yin",
note = "Funding Information: The authors thank W. Shih and P. Rothemund for helpful discussions. B.A. thanks A. Berliner, E. Groban, J. Schaeffer, F. Mazzoldi, A. Kimoto, L. Peck, and M. Tinnus for help with software tool development. The work was funded by Office of Naval Research grants N000141010827, N000141310593, N000141410610, N000141612182, and N000141612410; Army Research Office grant W911NF1210238; National Science Foundation grants CCF1054898, CMMI1333215, CMMI1334109, CMMI1344915, and CCF1317291; and National Institutes of Health grant 1R01EB01865901 to P.Y.; Office of Naval Research grant N000141512689 and NSF grants 1360635, 1563799, and 1334109 to H.Y.; and internal support from Autodesk Life Sciences. B.W. thanks the China Scholarship Council (No.201506340048) for fellowship support. C.M. was funded by the Fannie and John Hertz Foundation. All data are reported in the main text and in the Supplementary Materials. A provisional U.S. patent has been filed based on this work. P.Y. is cofounder of Ultivue Inc. and NuProbe Global.",
year = "2017",
month = dec,
day = "15",
doi = "10.1126/science.aao2648",
language = "English (US)",
volume = "358",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6369",
}