Single-shot detection of bacterial endospores via coherent Raman spectroscopy

Dmitry Pestov, Xi Wang, Gombojav O. Ariunbold, Robert K. Murawski, Vladimir A. Sautenkov, Arthur Dogariu, Alexei V. Sokolov, Marlan O. Scully

Research output: Contribution to journalArticlepeer-review

126 Scopus citations


Recent advances in coherent Raman spectroscopy hold exciting promise for many potential applications. For example, a technique, mitigating the nonresonant four-wave-mixing noise while maximizing the Raman-resonant signal, has been developed and applied to the problem of real-time detection of bacterial endospores. After a brief review of the technique essentials, we show how extensions of our earlier experimental work [Pestov D, et al. (2007) Science 316:265-268] yield single-shot identification of a small sample of Bacillus subtilis endospores (≈104 spores). The results convey the utility of the technique and its potential for "on-the-fly" detection of biohazards, such as Bacillus anthracis. The application of optimized coherent anti-Stokes Raman scattering scheme to problems requiring chemical specificity and short signal acquisition times is demonstrated.

Original languageEnglish (US)
Pages (from-to)422-427
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number2
StatePublished - Jan 15 2008

All Science Journal Classification (ASJC) codes

  • General


  • Biohazard
  • CARS
  • Hybrid
  • Identification
  • Technique


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