Single-Particle Cryo-Electron Microscopy: Mathematical Theory, Computational Challenges, and Opportunities

In recent years, an abundance of new molecular structures have been elucidated using cryo-electron microscopy (cryo-EM), largely due to advances in hardware technology and data processing techniques. Owing to these exciting new developments, cryo-EM was selected by Nature Methods as the "Method of the Year 2015," and the Nobel Prize in Chemistry 2017 was awarded to three pioneers in the cryo-EM field: Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryoelectron microscopy for the high-resolution structure determination of biomolecules in solution" [93].