Single mRNA molecule detection in drosophila

Shawn C. Little, Thomas Gregor

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations

Abstract

Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages127-142
Number of pages16
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1649
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Drosophila
  • FISH
  • Fluorescence imaging
  • Quantitation
  • Single molecule

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