Single mRNA molecule detection in drosophila

Shawn C. Little, Thomas Gregor

Research output: Chapter in Book/Report/Conference proceedingChapter

16 Scopus citations


Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages16
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology


  • Drosophila
  • FISH
  • Fluorescence imaging
  • Quantitation
  • Single molecule


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