Single-molecule studies of repressor-DNA interactions show long-range interactions

Y. M. Wang; Jonas O. Tegenfeldt; W. Reisner; R. Riehn; Xiao Juan Guan; Ling Guo; Ido Golding; Edward C. Cox; James Sturm; Robert H. Austin

doi:10.1073/pnas.0502917102

Single-molecule studies of repressor-DNA interactions show long-range interactions

Y. M. Wang, Jonas O. Tegenfeldt, W. Reisner, R. Riehn, Xiao Juan Guan, Ling Guo, Ido Golding, Edward C. Cox, James Sturm, Robert H. Austin

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

We have performed single-molecule studies of GFP-Lacl repressor proteins bound to bacteriophage λ DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to the 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.

Original language	English (US)
Pages (from-to)	9796-9801
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	28
DOIs	https://doi.org/10.1073/pnas.0502917102
State	Published - Jul 12 2005

All Science Journal Classification (ASJC) codes

General

Keywords

Binding coefficient
Multiple sites
Transcription factors

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10.1073/pnas.0502917102

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title = "Single-molecule studies of repressor-DNA interactions show long-range interactions",

abstract = "We have performed single-molecule studies of GFP-Lacl repressor proteins bound to bacteriophage λ DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to the 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.",

keywords = "Binding coefficient, Multiple sites, Transcription factors",

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T1 - Single-molecule studies of repressor-DNA interactions show long-range interactions

AU - Wang, Y. M.

AU - Tegenfeldt, Jonas O.

AU - Reisner, W.

AU - Riehn, R.

AU - Guan, Xiao Juan

AU - Guo, Ling

AU - Golding, Ido

AU - Cox, Edward C.

AU - Sturm, James

AU - Austin, Robert H.

PY - 2005/7/12

Y1 - 2005/7/12

N2 - We have performed single-molecule studies of GFP-Lacl repressor proteins bound to bacteriophage λ DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to the 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.

AB - We have performed single-molecule studies of GFP-Lacl repressor proteins bound to bacteriophage λ DNA containing a 256 tandem lac operator insertion confined in nanochannels. An integrated photon molecular counting method was developed to determine the number of proteins bound to DNA. By using this method, we determined the saturated mean occupancy of the 256 tandem lac operators to the 13, which constitutes only 2.5% of the available sites. This low occupancy level suggests that the repressors influence each other even when they are widely separated, at distances on the order of 200 nm, or several DNA persistence lengths.

KW - Binding coefficient

KW - Multiple sites

KW - Transcription factors

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Single-molecule studies of repressor-DNA interactions show long-range interactions

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