Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

David Liao; Peter Galajda; Robert Riehn; Rob Ilic; Jason L. Puchalla; Howard G. Yu; Harold G. Craighead; Robert H. Austin

doi:10.1364/OE.16.010077

Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

David Liao, Peter Galajda, Robert Riehn, Rob Ilic, Jason L. Puchalla, Howard G. Yu, Harold G. Craighead, Robert H. Austin

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δk_ON/k_ON| = |δk_OFF/k _OFF| ∼ k_ON+k_OFF/k_ON 0.1D _B/D_F-D_B e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.

Original language	English (US)
Pages (from-to)	10077-10090
Number of pages	14
Journal	Optics Express
Volume	16
Issue number	14
DOIs	https://doi.org/10.1364/OE.16.010077
State	Published - Jul 7 2008

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OE.16.010077

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title = "Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides",

abstract = "Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δkON/kON| = |δkOFF/k OFF| ∼ kON+kOFF/kON 0.1D B/DF-DB e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.",

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AU - Liao, David

AU - Galajda, Peter

AU - Riehn, Robert

AU - Ilic, Rob

AU - Puchalla, Jason L.

AU - Yu, Howard G.

AU - Craighead, Harold G.

AU - Austin, Robert H.

PY - 2008/7/7

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N2 - Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δkON/kON| = |δkOFF/k OFF| ∼ kON+kOFF/kON 0.1D B/DF-DB e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.

AB - Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δkON/kON| = |δkOFF/k OFF| ∼ kON+kOFF/kON 0.1D B/DF-DB e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.

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Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

Abstract

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