Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides

David Liao, Peter Galajda, Robert Riehn, Rob Ilic, Jason L. Puchalla, Howard G. Yu, Harold G. Craighead, Robert H. Austin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to ∼ 17 mm/s. We derive an analytic scaling relationship |δkON/kON| = |δkOFF/k OFF| ∼ kON+kOFF/kON 0.1D B/DF-DB e/√SNR converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.

Original languageEnglish (US)
Pages (from-to)10077-10090
Number of pages14
JournalOptics Express
Volume16
Issue number14
DOIs
StatePublished - Jul 7 2008

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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    Liao, D., Galajda, P., Riehn, R., Ilic, R., Puchalla, J. L., Yu, H. G., Craighead, H. G., & Austin, R. H. (2008). Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides. Optics Express, 16(14), 10077-10090. https://doi.org/10.1364/OE.16.010077