Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome

Florian Douam, Gabriela Hrebikova, Yentli E.Soto Albrecht, Julie Sellau, Yael Sharon, Qiang Ding, Alexander Ploss

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Positive-sense RNA viruses pose increasing health and economic concerns worldwide. Our limited understanding of how these viruses interact with their host and how these processes lead to virulence and disease seriously hampers the development of anti-viral strategies. Here, we demonstrate the tracking of (+) and (â) sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models. Our results provide insights into how a prototypic flavivirus, yellow fever virus (YFV-17D), differentially interacts with murine and human hematopoietic cells in these mouse models and how these dynamics influence distinct outcomes of infection. We detect (â) YFV-17D RNA in specific secondary lymphoid compartments and cell subsets not previously recognized as permissive for YFV replication, and we highlight potential virus-host interaction events that could be pivotal in regulating flavivirus virulence and attenuation.

Original languageEnglish (US)
Article number14781
JournalNature communications
Volume8
DOIs
StatePublished - Mar 14 2017

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Single-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome'. Together they form a unique fingerprint.

  • Cite this