Simultaneous imaging of multiple focal planes for three-dimensional microscopy using ultra-high-speed adaptive optics

Martí Duocastella, Bo Sun, Craig B. Arnold

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Traditional white-light and fluorescent imaging techniques provide powerful methods to extract high-resolution information from two-dimensional (2-D) sections, but to retrieve information from a three-dimensional (3-D) volume they require relatively slow scanning methods that result in increased acquisition time. Using an ultra-high speed liquid lens, we circumvent this problem by simultaneously acquiring images from multiple focal planes. We demonstrate this method by imaging microparticles and cells flowing in 3-D microfluidic channels.

Original languageEnglish (US)
Article number050505
JournalJournal of Biomedical Optics
Volume17
Issue number5
DOIs
StatePublished - May 1 2012

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Keywords

  • Adaptive optics
  • Cells
  • Microfluidics
  • Microscopy
  • Real-time imaging

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