Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields

John Peter Rickgauer, Karl Deisseroth, David W. Tank

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron's coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, l = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, l = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or 'biasing', to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields.

Original languageEnglish (US)
Pages (from-to)1816-1824
Number of pages9
JournalNature neuroscience
Volume17
Issue number12
DOIs
StatePublished - Jan 1 2014

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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