TY - JOUR
T1 - Simultaneous cellular-resolution optical perturbation and imaging of place cell firing fields
AU - Rickgauer, John Peter
AU - Deisseroth, Karl
AU - Tank, David W.
N1 - Funding Information:
We thank D. Kim and C. Guo (Genetically Encoded Neuronal Indicator and Effector Project, Janelia Research Campus) for transgenic mice, D. Aronov for VR software, B. Scott for discussions, and C. Domnisoru, A. Miri, F. Collman and S. Wang for comments on the manuscript. This work was supported by the US National Institutes of Health (R01-MH083686; P50-GM071508) and a National Science Foundation Graduate Research Fellowship to J.P.R.
Publisher Copyright:
© 2014 Nature America, Inc. All rights reserved.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron's coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, l = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, l = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or 'biasing', to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields.
AB - Linking neural microcircuit function to emergent properties of the mammalian brain requires fine-scale manipulation and measurement of neural activity during behavior, where each neuron's coding and dynamics can be characterized. We developed an optical method for simultaneous cellular-resolution stimulation and large-scale recording of neuronal activity in behaving mice. Dual-wavelength two-photon excitation allowed largely independent functional imaging with a green fluorescent calcium sensor (GCaMP3, l = 920 ± 6 nm) and single-neuron photostimulation with a red-shifted optogenetic probe (C1V1, l = 1,064 ± 6 nm) in neurons coexpressing the two proteins. We manipulated task-modulated activity in individual hippocampal CA1 place cells during spatial navigation in a virtual reality environment, mimicking natural place-field activity, or 'biasing', to reveal subthreshold dynamics. Notably, manipulating single place-cell activity also affected activity in small groups of other place cells that were active around the same time in the task, suggesting a functional role for local place cell interactions in shaping firing fields.
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U2 - 10.1038/nn.3866
DO - 10.1038/nn.3866
M3 - Article
C2 - 25402854
AN - SCOPUS:84925223053
SN - 1097-6256
VL - 17
SP - 1816
EP - 1824
JO - Nature Neuroscience
JF - Nature Neuroscience
IS - 12
ER -