Significance analysis of time course microarray experiments

John D. Storey, Wenzhong Xiao, Jeffrey T. Leek, Ronald G. Tompkins, Ronald W. Davis

Research output: Contribution to journalArticle

410 Scopus citations

Abstract

Characterizing the genome-wide dynamic regulation of gene expression is important and will be of much interest in the future. However, there is currently no established method for identifying differentially expressed genes in a time course study. Here we propose a significance method for analyzing time course microarray studies that can be applied to the typical types of comparisons and sampling schemes. This method is applied to two studies on humans. In one study, genes are identified that show differential expression over time in response to in vivo endotoxin administration. By using our method, 7,409 genes are called significant at a 1 % false-discovery rate level, whereas several existing approaches fail to identify any genes. In another study, 417 genes are identified at a 10% false-discovery rate level that show expression changing with age in the kidney cortex. Here it is also shown that as many as 47% of the genes change with age in a manner more complex than simple exponential growth or decay. The methodology proposed here has been implemented in the freely distributed and open-source EDGE software package.

Original languageEnglish (US)
Pages (from-to)12837-12842
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number36
DOIs
StatePublished - Sep 6 2005

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Aging
  • Differential expression
  • Expression arrays
  • Q values
  • Time series

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