Serial two-photon tomography for automated ex vivo mouse brain imaging

Timothy Ragan; Lolahon R. Kadiri; Kannan Umadevi Venkataraju; Karsten Bahlmann; Jason Sutin; Julian Taranda; Ignacio Arganda-Carreras; Yongsoo Kim; Hyunjune Sebastian Seung; Pavel Osten

doi:10.1038/nmeth.1854

Serial two-photon tomography for automated ex vivo mouse brain imaging

Timothy Ragan, Lolahon R. Kadiri, Kannan Umadevi Venkataraju, Karsten Bahlmann, Jason Sutin, Julian Taranda, Ignacio Arganda-Carreras, Yongsoo Kim, Hyunjune Sebastian Seung, Pavel Osten

Research output: Contribution to journal › Article › peer-review

328 Scopus citations

Abstract

Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

Original language	English (US)
Pages (from-to)	255-258
Number of pages	4
Journal	Nature Methods
Volume	9
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.1854
State	Published - Mar 2012

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1854

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title = "Serial two-photon tomography for automated ex vivo mouse brain imaging",

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Serial two-photon tomography for automated ex vivo mouse brain imaging. / Ragan, Timothy; Kadiri, Lolahon R.; Venkataraju, Kannan Umadevi; Bahlmann, Karsten; Sutin, Jason; Taranda, Julian; Arganda-Carreras, Ignacio; Kim, Yongsoo; Seung, Hyunjune Sebastian; Osten, Pavel.

In: Nature Methods, Vol. 9, No. 3, 03.2012, p. 255-258.

Research output: Contribution to journal › Article › peer-review

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