@article{6fd7056d732e440fb109e9634360e875,
title = "Serial two-photon tomography for automated ex vivo mouse brain imaging",
abstract = "Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.",
author = "Timothy Ragan and Kadiri, {Lolahon R.} and Venkataraju, {Kannan Umadevi} and Karsten Bahlmann and Jason Sutin and Julian Taranda and Ignacio Arganda-Carreras and Yongsoo Kim and Seung, {Hyunjune Sebastian} and Pavel Osten",
note = "Funding Information: We thank Y. Bao and R. Palaniswamy for expert technical assistance, S.C. Chen and M. Culpepper for design of the flexure-based microtome, Z.J. Huang (Cold Spring Harbor Laboratory) for the Sst-IRES-Cre{\o}Ai9 mouse, W. Denk, K. Rockland and F. Scalia for critical reading of the manuscript, and J. Kuhl for preparing art graphics and animation. This work was supported by Simons Foundation grant (137480) to P.O., McKnight Technological Innovations in Neuroscience Award to P.O., Howard Hughes Medical Institute Collaborative Innovation ward 43667 to H.S.S., and US National Institutes of Health grants 1 R43 HL093897-01 to T.R., R43 CA097670-01 and R44 CA097670-02 to T.R., K.B. and J.S.",
year = "2012",
month = mar,
doi = "10.1038/nmeth.1854",
language = "English (US)",
volume = "9",
pages = "255--258",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "3",
}