Serial two-photon tomography for automated ex vivo mouse brain imaging

Timothy Ragan, Lolahon R. Kadiri, Kannan Umadevi Venkataraju, Karsten Bahlmann, Jason Sutin, Julian Taranda, Ignacio Arganda-Carreras, Yongsoo Kim, Hyunjune Sebastian Seung, Pavel Osten

Research output: Contribution to journalArticlepeer-review

472 Scopus citations


Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
JournalNature Methods
Issue number3
StatePublished - Mar 2012

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Biochemistry
  • Biotechnology
  • Cell Biology


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