Sequencing in nanofabricated arrays: A feasibility study

Thomas Duke, Glen Monnelly, Robert H. Austin, Edward C. Cox

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The feasibility of using nanofabricated arrays as electrophoretic chambers for DNA sequencing is investigated. A specific array design, consisting of rows of closely spaced posts, separated by longer open spaces, is proposed. Molecules driven through the array by an electric field get hooked over obstacles at successive rows and their progress through the device is delayed as a consequence. The dependence of the delay time on molecular size is derived. Numerical evaluation indicates that a device of modest dimensions, operating at high fields, can rapidly resolve oligonucleotides containing several hundred bases.

Original languageEnglish (US)
Pages (from-to)17-22
Number of pages6
JournalELECTROPHORESIS
Volume18
Issue number1
DOIs
StatePublished - Jan 1997

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Keywords

  • DNA sequencing
  • Electrophoretic mobility
  • Microfabrication

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