Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs

Michael Grunstein; Paul Schedl; Laurence Kedes

doi:10.1016/0022-2836(76)90276-X

Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs

Michael Grunstein, Paul Schedl, Laurence Kedes

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

The putative histone H4 (F2a₁) mRNA has been isolated from early blastula Strongylocentrotus purpuratus sea urchin embryos. Nucleotide sequences of oligonucleotides obtained by digestion of this RNA with T₁ ribonuclease have been obtained and many are found to be colinear with the amino acid sequence of histone H4 protein. The sequences obtained from the H4 mRNAs of S. pnrpuratus have been compared with those obtained from Lytechinus pictus (Grunstein & Schedl, 1976). The two mRNAs for this highly conserved protein have undergone considerable divergence of the sort that would be predicted from the degeneracy of the genetic code. 11.5% of the bases have undergone substitution at a rate calculated to be 3 × 10^-9 base changes · codon^-1 · year^-1.

Original language	English (US)
Pages (from-to)	351-369
Number of pages	19
Journal	Journal of Molecular Biology
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/0022-2836(76)90276-X
State	Published - Jun 25 1976
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Biophysics
Structural Biology

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10.1016/0022-2836(76)90276-X

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title = "Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs",

abstract = "The putative histone H4 (F2a1) mRNA has been isolated from early blastula Strongylocentrotus purpuratus sea urchin embryos. Nucleotide sequences of oligonucleotides obtained by digestion of this RNA with T1 ribonuclease have been obtained and many are found to be colinear with the amino acid sequence of histone H4 protein. The sequences obtained from the H4 mRNAs of S. pnrpuratus have been compared with those obtained from Lytechinus pictus (Grunstein & Schedl, 1976). The two mRNAs for this highly conserved protein have undergone considerable divergence of the sort that would be predicted from the degeneracy of the genetic code. 11.5% of the bases have undergone substitution at a rate calculated to be 3 × 10-9 base changes · codon-1 · year-1.",

author = "Michael Grunstein and Paul Schedl and Laurence Kedes",

note = "Funding Information: This work was supported in part by research grants from the National Institutes of Health and the Veterans Administration. One of us (M. G.) was a fellow of the Leukemia Society of America. Another author (P. S.) was supported by a U.S. Public Health Service Biochemistry Training grant. The third author (L. K.) was a Scholar of the Leukemia Society of America and is an Investigator of the Howard Hughes Medical Institute.",

year = "1976",

month = jun,

day = "25",

doi = "10.1016/0022-2836(76)90276-X",

language = "English (US)",

volume = "104",

pages = "351--369",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

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T1 - Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs

AU - Grunstein, Michael

AU - Schedl, Paul

AU - Kedes, Laurence

N1 - Funding Information: This work was supported in part by research grants from the National Institutes of Health and the Veterans Administration. One of us (M. G.) was a fellow of the Leukemia Society of America. Another author (P. S.) was supported by a U.S. Public Health Service Biochemistry Training grant. The third author (L. K.) was a Scholar of the Leukemia Society of America and is an Investigator of the Howard Hughes Medical Institute.

PY - 1976/6/25

Y1 - 1976/6/25

N2 - The putative histone H4 (F2a1) mRNA has been isolated from early blastula Strongylocentrotus purpuratus sea urchin embryos. Nucleotide sequences of oligonucleotides obtained by digestion of this RNA with T1 ribonuclease have been obtained and many are found to be colinear with the amino acid sequence of histone H4 protein. The sequences obtained from the H4 mRNAs of S. pnrpuratus have been compared with those obtained from Lytechinus pictus (Grunstein & Schedl, 1976). The two mRNAs for this highly conserved protein have undergone considerable divergence of the sort that would be predicted from the degeneracy of the genetic code. 11.5% of the bases have undergone substitution at a rate calculated to be 3 × 10-9 base changes · codon-1 · year-1.

AB - The putative histone H4 (F2a1) mRNA has been isolated from early blastula Strongylocentrotus purpuratus sea urchin embryos. Nucleotide sequences of oligonucleotides obtained by digestion of this RNA with T1 ribonuclease have been obtained and many are found to be colinear with the amino acid sequence of histone H4 protein. The sequences obtained from the H4 mRNAs of S. pnrpuratus have been compared with those obtained from Lytechinus pictus (Grunstein & Schedl, 1976). The two mRNAs for this highly conserved protein have undergone considerable divergence of the sort that would be predicted from the degeneracy of the genetic code. 11.5% of the bases have undergone substitution at a rate calculated to be 3 × 10-9 base changes · codon-1 · year-1.

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JF - Journal of Molecular Biology

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Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs

Abstract

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