Sequence analysis and evolution of sea urchin (Lytechinus pictus and Strongylocentrotus purpuratus) histone H4 messenger RNAs

Michael Grunstein, Paul Daniel Schedl, Laurence Kedes

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The putative histone H4 (F2a1) mRNA has been isolated from early blastula Strongylocentrotus purpuratus sea urchin embryos. Nucleotide sequences of oligonucleotides obtained by digestion of this RNA with T1 ribonuclease have been obtained and many are found to be colinear with the amino acid sequence of histone H4 protein. The sequences obtained from the H4 mRNAs of S. pnrpuratus have been compared with those obtained from Lytechinus pictus (Grunstein & Schedl, 1976). The two mRNAs for this highly conserved protein have undergone considerable divergence of the sort that would be predicted from the degeneracy of the genetic code. 11.5% of the bases have undergone substitution at a rate calculated to be 3 × 10-9 base changes · codon-1 · year-1.

Original languageEnglish (US)
Pages (from-to)351-369
Number of pages19
JournalJournal of Molecular Biology
Volume104
Issue number2
DOIs
StatePublished - Jun 25 1976
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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