Separation of 100-kilobase DNA molecules in 10 seconds

O. Bakajin, T. A.J. Duke, J. Tegenfeldt, C. F. Chou, S. S. Chan, R. H. Austin, E. C. Cox

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

Original languageEnglish (US)
Pages (from-to)6053-6056
Number of pages4
JournalAnalytical Chemistry
Volume73
Issue number24
DOIs
StatePublished - Dec 15 2001

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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