Separation of 100-kilobase DNA molecules in 10 seconds

O. Bakajin; T. A.J. Duke; J. Tegenfeldt; C. F. Chou; S. S. Chan; R. H. Austin; E. C. Cox

doi:10.1021/ac015527o

Separation of 100-kilobase DNA molecules in 10 seconds

O. Bakajin, T. A.J. Duke, J. Tegenfeldt, C. F. Chou, S. S. Chan, R. H. Austin, E. C. Cox

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

Original language	English (US)
Pages (from-to)	6053-6056
Number of pages	4
Journal	Analytical Chemistry
Volume	73
Issue number	24
DOIs	https://doi.org/10.1021/ac015527o
State	Published - Dec 15 2001

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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10.1021/ac015527o

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title = "Separation of 100-kilobase DNA molecules in 10 seconds",

abstract = "Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.",

author = "O. Bakajin and Duke, {T. A.J.} and J. Tegenfeldt and Chou, {C. F.} and Chan, {S. S.} and Austin, {R. H.} and Cox, {E. C.}",

year = "2001",

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doi = "10.1021/ac015527o",

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journal = "Analytical Chemistry",

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AU - Bakajin, O.

AU - Duke, T. A.J.

AU - Tegenfeldt, J.

AU - Chou, C. F.

AU - Chan, S. S.

AU - Austin, R. H.

AU - Cox, E. C.

PY - 2001/12/15

Y1 - 2001/12/15

N2 - Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

AB - Long double-stranded DNA molecules were separated in microfabricated hexagonal arrays in less than 1 min, several orders of magnitude faster than by using conventional technology. DNA samples were first concentrated at the entrance to the array in a thin band by entropic focusing. They were then separated by pulsed field electrophoresis. T4 (168.9 kbp) and λ (48.5 kbp) DNAs could be resolved into two clearly separated bands in ∼10 s in these experiments. This corresponds to a mass resolution of 6% in 11 min in a 1-cm-long array.

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EP - 6056

JO - Analytical Chemistry

JF - Analytical Chemistry

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ER -

Separation of 100-kilobase DNA molecules in 10 seconds

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