Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry

Sunil U. Bajad; Wenyun Lu; Elizabeth H. Kimball; Jie Yuan; Celeste Peterson; Joshua D. Rabinowitz

doi:10.1016/j.chroma.2006.05.019

Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry

Sunil U. Bajad, Wenyun Lu, Elizabeth H. Kimball, Jie Yuan, Celeste Peterson, Joshua D. Rabinowitz

Research output: Contribution to journal › Article › peer-review

405 Scopus citations

Abstract

A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic interaction chromatography with an amino column, effectively separates highly water soluble metabolites that fail to retain using standard reversed-phase chromatography. MS/MS detection is achieved by scanning through numerous selected reaction monitoring events on a triple quadrupole instrument. When applied to extracts of Escherichia coli grown in [¹²C]- versus [¹³C]glucose, the method reveals appropriate ¹²C- and ¹³C-peaks for 79 different metabolites.

Original language	English (US)
Pages (from-to)	76-88
Number of pages	13
Journal	Journal of Chromatography A
Volume	1125
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2006.05.019
State	Published - Aug 25 2006

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

Keywords

Bacteria
Escherichia coli
Hydrophilic interaction chromatography
LC-ESI-MS/MS
Mass spectrometry
Metabolite
Metabolomics
Metabonomics
Triple quadrupole
metabolism

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10.1016/j.chroma.2006.05.019

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title = "Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry",

abstract = "A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic interaction chromatography with an amino column, effectively separates highly water soluble metabolites that fail to retain using standard reversed-phase chromatography. MS/MS detection is achieved by scanning through numerous selected reaction monitoring events on a triple quadrupole instrument. When applied to extracts of Escherichia coli grown in [12C]- versus [13C]glucose, the method reveals appropriate 12C- and 13C-peaks for 79 different metabolites.",

keywords = "Bacteria, Escherichia coli, Hydrophilic interaction chromatography, LC-ESI-MS/MS, Mass spectrometry, Metabolite, Metabolomics, Metabonomics, Triple quadrupole, metabolism",

author = "Bajad, {Sunil U.} and Wenyun Lu and Kimball, {Elizabeth H.} and Jie Yuan and Celeste Peterson and Rabinowitz, {Joshua D.}",

note = "Funding Information: We thank David Botstein, Ned Wingreen, Leonid Kruglyak, Thomas Silhavy, John T. Groves, Sanford Silverman, Matthew Brauer, Melisa Gao, and Haoqian Chen for their support and technical and intellectual contributions to this research. Thanks also to the Lewis-Sigler Institute, Princeton University Department of Chemistry, and the National Institutes of Health, and the Beckman Foundation for funding these efforts. ",

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doi = "10.1016/j.chroma.2006.05.019",

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AU - Bajad, Sunil U.

AU - Lu, Wenyun

AU - Kimball, Elizabeth H.

AU - Yuan, Jie

AU - Peterson, Celeste

AU - Rabinowitz, Joshua D.

N1 - Funding Information: We thank David Botstein, Ned Wingreen, Leonid Kruglyak, Thomas Silhavy, John T. Groves, Sanford Silverman, Matthew Brauer, Melisa Gao, and Haoqian Chen for their support and technical and intellectual contributions to this research. Thanks also to the Lewis-Sigler Institute, Princeton University Department of Chemistry, and the National Institutes of Health, and the Beckman Foundation for funding these efforts.

PY - 2006/8/25

Y1 - 2006/8/25

N2 - A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic interaction chromatography with an amino column, effectively separates highly water soluble metabolites that fail to retain using standard reversed-phase chromatography. MS/MS detection is achieved by scanning through numerous selected reaction monitoring events on a triple quadrupole instrument. When applied to extracts of Escherichia coli grown in [12C]- versus [13C]glucose, the method reveals appropriate 12C- and 13C-peaks for 79 different metabolites.

AB - A key unmet need in metabolomics is the ability to efficiently quantify a large number of known cellular metabolites. Here we present a liquid chromatography (LC)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) method for reliable measurement of 141 metabolites, including components of central carbon, amino acid, and nucleotide metabolism. The selected LC approach, hydrophilic interaction chromatography with an amino column, effectively separates highly water soluble metabolites that fail to retain using standard reversed-phase chromatography. MS/MS detection is achieved by scanning through numerous selected reaction monitoring events on a triple quadrupole instrument. When applied to extracts of Escherichia coli grown in [12C]- versus [13C]glucose, the method reveals appropriate 12C- and 13C-peaks for 79 different metabolites.

KW - Bacteria

KW - Escherichia coli

KW - Hydrophilic interaction chromatography

KW - LC-ESI-MS/MS

KW - Mass spectrometry

KW - Metabolite

KW - Metabolomics

KW - Metabonomics

KW - Triple quadrupole

KW - metabolism

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SN - 0021-9673

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JO - Journal of Chromatography A

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ER -

Separation and quantitation of water soluble cellular metabolites by hydrophilic interaction chromatography-tandem mass spectrometry

Abstract

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