@article{ed16070ca11a468c9a433aa080c5cf55,
title = "Separating Functions of the Phage-Encoded Quorum-Sensing-Activated Antirepressor Qtip",
abstract = "Qtip is a 79 amino acid antirepressor that connects phage quorum sensing to host-cell lysis. Silpe and Bridges et al. demonstrate that Qtip binds, inactivates, and sequesters the phage cI repressor at the cell pole. Qtip mutagenesis reveals discrete sites required for repressor recognition and polar localization, showing that the two functions are separable.",
keywords = "Qtip, antirepressor, cI repressor, lysis-lysogeny, phage, quorum sensing",
author = "Silpe, {Justin E.} and Bridges, {Andrew A.} and Xiuliang Huang and Coronado, {Daniela R.} and Duddy, {Olivia P.} and Bassler, {Bonnie L.}",
note = "Funding Information: This work was supported by the Howard Hughes Medical Institute , NIH grant R37GM065859 , National Science Foundation grant MCB-1713731 (to B.L.B.), NIGMS grant T32GM007388 (O.P.D.), the Damon Runyon Fellowship Award DRG-2302-17 (to A.A.B.), a Charlotte Elizabeth Procter Fellowship provided by Princeton University (to J.E.S.), and a National Defense Science and Engineering Graduate Fellowship supported by the Department of Defense (to J.E.S.). We are grateful to Dr. Luke Lavis for providing ample SNAP-JF 503 ligand. We thank members of PulseNet and the National Salmonella Reference Laboratory for information on the reported Salmonella-derived contig. We thank Tom Silhavy for helpful discussions and Betsy Hart for the cI857 strain. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Funding Information: This work was supported by the Howard Hughes Medical Institute, NIH grant R37GM065859, National Science Foundation grant MCB-1713731 (to B.L.B.), NIGMS grant T32GM007388 (O.P.D.), the Damon Runyon Fellowship Award DRG-2302-17 (to A.A.B.), a Charlotte Elizabeth Procter Fellowship provided by Princeton University (to J.E.S.), and a National Defense Science and Engineering Graduate Fellowship supported by the Department of Defense (to J.E.S.). We are grateful to Dr. Luke Lavis for providing ample SNAP-JF503 ligand. We thank members of PulseNet and the National Salmonella Reference Laboratory for information on the reported Salmonella-derived contig. We thank Tom Silhavy for helpful discussions and Betsy Hart for the cI857 strain. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. J.E.S. D.R.C. and O.P.D. constructed strains; J.E.S. A.A.B. X.H. D.R.C. and O.P.D. performed experiments; J.E.S. A.A.B. X.H. O.P.D. and B.L.B. analyzed data; J.E.S. A.A.B. and B.L.B. designed experiments; and J.E.S. and B.L.B. wrote the paper. The authors declare no competing financial interests. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = apr,
day = "8",
doi = "10.1016/j.chom.2020.01.024",
language = "English (US)",
volume = "27",
pages = "629--641.e4",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "4",
}