@article{44577f2d65a14716a37de15942b0ae10,
title = "Sensory Discrimination of Blood and Floral Nectar by Aedes aegypti Mosquitoes",
abstract = "Jov{\'e} et al. characterize specialized blood-feeding sensory neurons in female Aedes aegypti mosquitoes and demonstrate that they encode the distinction between blood and floral nectar. Polymodal integrator neurons respond to glucose only in the presence of additional blood components, allowing mosquitoes to discriminate these two appetizing food sources.",
keywords = "Aedes aegypti, GCaMP calcium imaging, blood-feeding behavior, chemogenetics, gustatory receptors, ionotropic receptors, mosquito, nectar-feeding behavior, stylet, taste",
author = "Veronica Jov{\'e} and Zhongyan Gong and Hol, {Felix J.H.} and Zhilei Zhao and Sorrells, {Trevor R.} and Carroll, {Thomas S.} and Manu Prakash and McBride, {Carolyn S.} and Vosshall, {Leslie B.}",
note = "Funding Information: We thank J. Clowney, M. DeGennaro, E.J. Dennis, M. Dietrich, L. Duvall, A. Handler, R. Jov{\'e}, K.J. Lee, M.Z. Liu, B.J. Matthews, P. Muller, N. Yapici, and members of the Vosshall Lab for comments on the manuscript; T. Morita for guidance and technical assistance with stylet GCaMP calcium imaging; B. Matthews for advice regarding RNA-seq and transgenic strain design; L. Duvall for advice regarding Glytube assays; G. Gordon, L. Mejia, and A. Pandey for mosquito rearing; W. Freiwald, P. Muller, and V. Ruta for discussions; D. Gross and J. Petrillo for advice regarding imaging hardware; G. Jeffries of Fluicell for optimizing the BioPen; C. Zhao and B. Zhang for help with RNA-seq library preparation; A. North and C. Pyrgaki for help with confocal and structured illumination image acquisition; R. Harrell II at ITF for embryo injections; T. Siskoglou and A. Gerson of Morrell Instruments for help with imaging hardware; and B. Matthews, M. Younger, and the Aedes Toolkit Group for early access to unpublished strains. This work was supported in part by NCATS NIH CTSA grant UL1 TR000043, NIH T32-MH095246 (to V.J.), an HHMI Gilliam fellowship (to V.J.), NSF DGE-1325261 (to V.J.), the CASI/Burroughs Wellcome Fund (to F.J.J.H.), Marie Sk{\l}odowska-Curie grant agreement 841893 – PiQiMosqBite (to F.J.J.H.), a Jane Coffin Childs postdoctoral fellowship (to T.R.S.), a Kavli Neural Systems Institute postdoctoral fellowship (to T.R.S.), NIDCD R00-DC012069 (to C.S.M.), NIH DP2-AI124336 (to M.P.), and a USAID Grand Challenges: Zika and Future Threats award (to M.P.). M.P. is an HHMI-Gates Faculty Scholar of the Howard Hughes Medical Institute. C.S.M. is a New York Stem Cell Foundation – Robertson Investigator. L.B.V. is an investigator of the Howard Hughes Medical Institute. V.J. performed all experiments in the paper with the following exceptions: F.J.H.H. (Figure 2; Video 1), Z.G. (Figures 1E, 1F, 1J, S1D, 7B, and 7C), and T.R.S. (Figures 6C–6F, S7C, and S7D; Video 6). T.S.C. wrote the live imaging analysis pipeline code and performed statistical analyses for Figure S5. Z.G. and V.J. together created the Ir7a-QF2, Gr4-QF2, and QUAS-TRPV1 strains and performed experiments for Figures 1I, S1A–S1C, 3A, 3B, S2B, S2C, S2F, S2G, 6A, 6B, and 7E. Z.Z. and C.S.M. shared their unpublished Brp-QF2w strain. M.P. supervised and collaborated with F.J.H.H. V.J. and L.B.V. together conceived the study, designed the figures, and wrote the paper with input from all authors. The authors declare no competing interests. Funding Information: We thank J. Clowney, M. DeGennaro, E.J. Dennis, M. Dietrich, L. Duvall, A. Handler, R. Jov{\'e}, K.J. Lee, M.Z. Liu, B.J. Matthews, P. Muller, N. Yapici, and members of the Vosshall Lab for comments on the manuscript; T. Morita for guidance and technical assistance with stylet GCaMP calcium imaging; B. Matthews for advice regarding RNA-seq and transgenic strain design; L. Duvall for advice regarding Glytube assays; G. Gordon, L. Mejia, and A. Pandey for mosquito rearing; W. Freiwald, P. Muller, and V. Ruta for discussions; D. Gross and J. Petrillo for advice regarding imaging hardware; G. Jeffries of Fluicell for optimizing the BioPen; C. Zhao and B. Zhang for help with RNA-seq library preparation; A. North and C. Pyrgaki for help with confocal and structured illumination image acquisition; R. Harrell II at ITF for embryo injections; T. Siskoglou and A. Gerson of Morrell Instruments for help with imaging hardware; and B. Matthews, M. Younger, and the Aedes Toolkit Group for early access to unpublished strains. This work was supported in part by NCATS NIH CTSA grant UL1 TR000043 , NIH T32-MH095246 (to V.J.), an HHMI Gilliam fellowship (to V.J.), NSF DGE-1325261 (to V.J.), the CASI/Burroughs Wellcome Fund (to F.J.J.H.), Marie Sk{\l}odowska-Curie grant agreement 841893 – PiQiMosqBite (to F.J.J.H.), a Jane Coffin Childs postdoctoral fellowship (to T.R.S.), a Kavli Neural Systems Institute postdoctoral fellowship (to T.R.S.), NIDCD R00-DC012069 (to C.S.M.), NIH DP2-AI124336 (to M.P.), and a USAID Grand Challenges: Zika and Future Threats award (to M.P.). M.P. is an HHMI-Gates Faculty Scholar of the Howard Hughes Medical Institute. C.S.M. is a New York Stem Cell Foundation – Robertson Investigator. L.B.V. is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = dec,
day = "23",
doi = "10.1016/j.neuron.2020.09.019",
language = "English (US)",
volume = "108",
pages = "1163--1180.e12",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "6",
}