Semisynthesis of Phosphovariants of Smad2 Reveals a Substrate Preference of the Activated TβRI Kinase

Jennifer J. Ottesen, Morgan Huse, Matthew D. Sekedat, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Transforming growth factor-β (TGF-β) signaling regulates a wide range of cellular processes. Aberrant TGF-β signaling has been implicated in various disease states in humans. A key element in this signaling pathway is phosphorylation of R-Smads such as Smad2 at the last two serine residues of the C-terminal sequence CSSXS (residues 463-467 in Smad2) by the TβRI receptor kinase. Phosphorylation results in the release of the R-Smad from the membrane-anchored protein SARA, binding to the co-mediator protein Smad4, translocation into the nucleus, and regulation of target gene expression. Expressed protein ligation was used to probe the contribution of the individual phosphate groups to Smad2 oligomerization and phosphorylation by TβRI. Phosphorylation at both positions was required to generate a stable homotrimer; however, the driving force for Smad2 self-association is provided by pSer465. Additionally, SARA was found to modulate the self-association of partially phosphorylated Smad2, which suggests an added role for this protein in preventing premature release of a monophosphorylated substrate from the receptor complex. In related studies, prephosphorylation of Smad2 at Ser465 was found to significantly increase the rate of phosphorylation at Ser467, suggesting that there may be specific recognition determinants within the kinase for the monophosphorylated intermediate. This information was exploited to design an improved peptide substrate for TβRI, which may prove valuable in the design of inhibitors of the TGF-β pathway.

Original languageEnglish (US)
Pages (from-to)5698-5706
Number of pages9
JournalBiochemistry
Volume43
Issue number19
DOIs
StatePublished - May 18 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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