Selection of sequence elements that substitute for the standard AATAAA motif which signals 3′ processing and polyadenylation of late simian virus 40 mRNAs

Candace Swimmer, Thomas Shenk

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants wre characterized. All displayed wild-type growth kinetics and produced normal levels of late mRNAs and proteins. Two variants had reacquired AATAAA elements and one acquired an ATTAAA sequence. The last variant carried an ATTTTTTAAA segment, suggesting this novel sequence, or some portion of it, can also signal poly A addition.

Original languageEnglish (US)
Pages (from-to)8053-8063
Number of pages11
JournalNucleic acids research
Volume13
Issue number22
DOIs
StatePublished - Nov 25 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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