TY - JOUR
T1 - Selection of sequence elements that substitute for the standard AATAAA motif which signals 3′ processing and polyadenylation of late simian virus 40 mRNAs
AU - Swimmer, Candace
AU - Shenk, Thomas
N1 - Funding Information:
grant from the National Cancer Institute
PY - 1985/11/25
Y1 - 1985/11/25
N2 - A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants wre characterized. All displayed wild-type growth kinetics and produced normal levels of late mRNAs and proteins. Two variants had reacquired AATAAA elements and one acquired an ATTAAA sequence. The last variant carried an ATTTTTTAAA segment, suggesting this novel sequence, or some portion of it, can also signal poly A addition.
AB - A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants wre characterized. All displayed wild-type growth kinetics and produced normal levels of late mRNAs and proteins. Two variants had reacquired AATAAA elements and one acquired an ATTAAA sequence. The last variant carried an ATTTTTTAAA segment, suggesting this novel sequence, or some portion of it, can also signal poly A addition.
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U2 - 10.1093/nar/13.22.8053
DO - 10.1093/nar/13.22.8053
M3 - Article
C2 - 2866490
AN - SCOPUS:0022432575
SN - 0305-1048
VL - 13
SP - 8053
EP - 8063
JO - Nucleic acids research
JF - Nucleic acids research
IS - 22
ER -