Sec6 mutations and the Drosophila exocyst complex

Mala Murthy; Ravi Ranjan; Natalie Denef; Misao E.L. Higashi; Trudi Schupbach; Thomas L. Schwarz

doi:10.1242/jcs.01644

Sec6 mutations and the Drosophila exocyst complex

Mala Murthy, Ravi Ranjan, Natalie Denef, Misao E.L. Higashi, Trudi Schupbach, Thomas L. Schwarz

Molecular Biology

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

Original language	English (US)
Pages (from-to)	1139-1150
Number of pages	12
Journal	Journal of cell science
Volume	118
Issue number	6
DOIs	https://doi.org/10.1242/jcs.01644
State	Published - Mar 15 2005

All Science Journal Classification (ASJC) codes

Cell Biology

Keywords

Membrane trafficking
Oogenesis
Sec5
Sec6
Sec8

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10.1242/jcs.01644

Cite this

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title = "Sec6 mutations and the Drosophila exocyst complex",

abstract = "To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.",

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author = "Mala Murthy and Ravi Ranjan and Natalie Denef and Higashi, {Misao E.L.} and Trudi Schupbach and Schwarz, {Thomas L.}",

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T1 - Sec6 mutations and the Drosophila exocyst complex

AU - Murthy, Mala

AU - Ranjan, Ravi

AU - Denef, Natalie

AU - Higashi, Misao E.L.

AU - Schupbach, Trudi

AU - Schwarz, Thomas L.

PY - 2005/3/15

Y1 - 2005/3/15

N2 - To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

AB - To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

KW - Membrane trafficking

KW - Oogenesis

KW - Sec5

KW - Sec6

KW - Sec8

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Sec6 mutations and the Drosophila exocyst complex

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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