TY - JOUR
T1 - Saccharomyces telomeres acquire single-strand TG1-3 tails late in S phase
AU - Wellinger, Raymund J.
AU - Wolf, Alexander J.
AU - Zakian, Virginia A.
PY - 1993/1/15
Y1 - 1993/1/15
N2 - Saccharomyces telomeres consist of ∼ 300 bp of C1-3A TG1-3 DNA. Nondenaturing Southern hybridization, capable of detecting ∼60 to ∼300 bases of TG1-3 DNA, revealed that yeast telomeres acquired and lost TG1-3 tails, the predicted intermediate in telomere replication, in a cell cycle-dependent manner. TG1-3 tails were also detected on the ends of a linear plasmid isolated from late S phase cells. In addition, a nonlinear form of this plasmid was detected: this structure migrated in two-dimensional agarose gels like a nicked circle of the same size as the linear plasmid, but had considerably more single-stranded character than a conventional nicked circle. The evidence indicates that these circles were formed by telomere-telomere interactions involving the TG1-3 tails. These data provide evidence for a cell cycle-dependent change in telomere structure and demonstrate that TG1-3 tails, generated during replication of a linear plasmid in vivo, are capable of mediating telomere-telomere interactions.
AB - Saccharomyces telomeres consist of ∼ 300 bp of C1-3A TG1-3 DNA. Nondenaturing Southern hybridization, capable of detecting ∼60 to ∼300 bases of TG1-3 DNA, revealed that yeast telomeres acquired and lost TG1-3 tails, the predicted intermediate in telomere replication, in a cell cycle-dependent manner. TG1-3 tails were also detected on the ends of a linear plasmid isolated from late S phase cells. In addition, a nonlinear form of this plasmid was detected: this structure migrated in two-dimensional agarose gels like a nicked circle of the same size as the linear plasmid, but had considerably more single-stranded character than a conventional nicked circle. The evidence indicates that these circles were formed by telomere-telomere interactions involving the TG1-3 tails. These data provide evidence for a cell cycle-dependent change in telomere structure and demonstrate that TG1-3 tails, generated during replication of a linear plasmid in vivo, are capable of mediating telomere-telomere interactions.
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U2 - 10.1016/0092-8674(93)90049-V
DO - 10.1016/0092-8674(93)90049-V
M3 - Article
C2 - 8422682
AN - SCOPUS:0027509950
VL - 72
SP - 51
EP - 60
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -