Saccharomyces telomeres acquire single-strand TG1-3 tails late in S phase

Raymund J. Wellinger, Alexander J. Wolf, Virginia A. Zakian

Research output: Contribution to journalArticle

327 Scopus citations

Abstract

Saccharomyces telomeres consist of ∼ 300 bp of C1-3A TG1-3 DNA. Nondenaturing Southern hybridization, capable of detecting ∼60 to ∼300 bases of TG1-3 DNA, revealed that yeast telomeres acquired and lost TG1-3 tails, the predicted intermediate in telomere replication, in a cell cycle-dependent manner. TG1-3 tails were also detected on the ends of a linear plasmid isolated from late S phase cells. In addition, a nonlinear form of this plasmid was detected: this structure migrated in two-dimensional agarose gels like a nicked circle of the same size as the linear plasmid, but had considerably more single-stranded character than a conventional nicked circle. The evidence indicates that these circles were formed by telomere-telomere interactions involving the TG1-3 tails. These data provide evidence for a cell cycle-dependent change in telomere structure and demonstrate that TG1-3 tails, generated during replication of a linear plasmid in vivo, are capable of mediating telomere-telomere interactions.

Original languageEnglish (US)
Pages (from-to)51-60
Number of pages10
JournalCell
Volume72
Issue number1
DOIs
StatePublished - Jan 15 1993

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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