Abstract
In wild-type Saccharomyces cerevisiae, replication forks slowed during their passage through telomeric C1-3A/TG1-3 tracts. This slowing was greatly exacerbated in the absence of RRM3, shown here to encode a 5′ to 3′ DNA helicase. Rrm3p-dependent fork progression was seen at a modified Chromosome VII-L telomere, at the natural X-bearing Chromosome III-L telomere, and at Y′-bearing telomeres. Loss of Rrm3p also resulted in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C1-3A/TG1-3 DNA. The ATPase/helicase activity of Rrm3p was required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p was telomere-associated in vivo, it likely has a direct role in telomere replication.
Original language | English (US) |
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Pages (from-to) | 1383-1396 |
Number of pages | 14 |
Journal | Genes and Development |
Volume | 16 |
Issue number | 11 |
DOIs | |
State | Published - Jun 1 2002 |
All Science Journal Classification (ASJC) codes
- Genetics
- Developmental Biology
Keywords
- Helicase
- RRM3
- Replication
- Telomerase
- Telomere
- Yeast