Saccharomyces Rrm3p, a 5′ to 3′ DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA

Andreas S. Ivessa, Jin Qiu Zhou, Vince P. Schulz, Ellen K. Monson, Virginia A. Zakian

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

In wild-type Saccharomyces cerevisiae, replication forks slowed during their passage through telomeric C1-3A/TG1-3 tracts. This slowing was greatly exacerbated in the absence of RRM3, shown here to encode a 5′ to 3′ DNA helicase. Rrm3p-dependent fork progression was seen at a modified Chromosome VII-L telomere, at the natural X-bearing Chromosome III-L telomere, and at Y′-bearing telomeres. Loss of Rrm3p also resulted in replication fork pausing at specific sites in subtelomeric DNA, such as at inactive replication origins, and at internal tracts of C1-3A/TG1-3 DNA. The ATPase/helicase activity of Rrm3p was required for its role in telomeric and subtelomeric DNA replication. Because Rrm3p was telomere-associated in vivo, it likely has a direct role in telomere replication.

Original languageEnglish (US)
Pages (from-to)1383-1396
Number of pages14
JournalGenes and Development
Volume16
Issue number11
DOIs
StatePublished - Jun 1 2002

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • Helicase
  • RRM3
  • Replication
  • Telomerase
  • Telomere
  • Yeast

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