S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association

Lara K. Goudsouzian, Creighton T. Tuzon, Virginia A. Zakian

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

In diverse organisms, the Mre11 complex and phosphoinositide 3-kinase-related kinases (PIKKs), such as Tel1p and Mec1p from S. cerevisiae, are key mediators of DNA repair and DNA damage checkpoints that also function at telomeres. Here, we use chromatin immunoprecipitation (ChIP) to determine if Mre11p, Tel1p, or Mec1p affects telomere maintenance by promoting recruitment of telomerase subunits to S. cerevisiae telomeres. We find that recruitment of Est2p, the catalytic subunit of telomerase, and Est1p, a telomerase accessory protein, was severely reduced in mre11Δ and tel1Δ cells. In contrast, the levels of Est2p and Est1p binding in late S/G2 phase, the period in the cell cycle when yeast telomerase lengthens telomeres, were indistinguishable in wild-type (WT) and mec1D cells. These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell.

Original languageEnglish (US)
Pages (from-to)603-610
Number of pages8
JournalMolecular Cell
Volume24
Issue number4
DOIs
StatePublished - Nov 17 2006

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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