TY - JOUR
T1 - S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association
AU - Goudsouzian, Lara K.
AU - Tuzon, Creighton T.
AU - Zakian, Virginia A.
N1 - Funding Information:
We thank A. Matsuura for providing primer sequences and PCR conditions and I. Cheung, J. Phillips, and M. Sabourin for comments on the manuscript. This work was supported by US National Institutes of Health (NIH) grant GM43265. L.K.G. was supported in part by NIH training grant T32 CA09528.
PY - 2006/11/17
Y1 - 2006/11/17
N2 - In diverse organisms, the Mre11 complex and phosphoinositide 3-kinase-related kinases (PIKKs), such as Tel1p and Mec1p from S. cerevisiae, are key mediators of DNA repair and DNA damage checkpoints that also function at telomeres. Here, we use chromatin immunoprecipitation (ChIP) to determine if Mre11p, Tel1p, or Mec1p affects telomere maintenance by promoting recruitment of telomerase subunits to S. cerevisiae telomeres. We find that recruitment of Est2p, the catalytic subunit of telomerase, and Est1p, a telomerase accessory protein, was severely reduced in mre11Δ and tel1Δ cells. In contrast, the levels of Est2p and Est1p binding in late S/G2 phase, the period in the cell cycle when yeast telomerase lengthens telomeres, were indistinguishable in wild-type (WT) and mec1D cells. These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell.
AB - In diverse organisms, the Mre11 complex and phosphoinositide 3-kinase-related kinases (PIKKs), such as Tel1p and Mec1p from S. cerevisiae, are key mediators of DNA repair and DNA damage checkpoints that also function at telomeres. Here, we use chromatin immunoprecipitation (ChIP) to determine if Mre11p, Tel1p, or Mec1p affects telomere maintenance by promoting recruitment of telomerase subunits to S. cerevisiae telomeres. We find that recruitment of Est2p, the catalytic subunit of telomerase, and Est1p, a telomerase accessory protein, was severely reduced in mre11Δ and tel1Δ cells. In contrast, the levels of Est2p and Est1p binding in late S/G2 phase, the period in the cell cycle when yeast telomerase lengthens telomeres, were indistinguishable in wild-type (WT) and mec1D cells. These data argue that Mre11p and Tel1p affect telomere length by promoting telomerase recruitment to telomeres, whereas Mec1p has only a minor role in telomerase recruitment in a TEL1 cell.
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U2 - 10.1016/j.molcel.2006.10.005
DO - 10.1016/j.molcel.2006.10.005
M3 - Article
C2 - 17188035
AN - SCOPUS:34547813672
SN - 1097-2765
VL - 24
SP - 603
EP - 610
JO - Molecular Cell
JF - Molecular Cell
IS - 4
ER -