TY - JOUR
T1 - RpoS proteolysis is controlled directly by ATP levels in Escherichia coli
AU - Peterson, Celeste N.
AU - Levchenko, Igor
AU - Rabinowitz, Joshua D.
AU - Baker, Tania A.
AU - Silhavy, Thomas J.
PY - 2012/3/15
Y1 - 2012/3/15
N2 - The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degradation in vivo. Nutrient upshifts trigger RpoS degradation independently of protein synthesis by activating metabolic pathways that generate small energy molecules. Using metabolic mutants and inhibitors, we show that ATP, but not GTP or NADH, is necessary for RpoS degradation. In vitro reconstitution assays directly demonstrate that ClpXP fails to degrade RpoS, but not other proteins, at low ATP hydrolysis rates. These data suggest that cellular ATP levels directly control RpoS stability.
AB - The master regulator of stationary phase in Escherichia coli, RpoS, responds to carbon availability through changes in stability, but the individual steps in the pathway are unknown. Here we systematically block key steps of glycolysis and the citric acid cycle and monitor the effect on RpoS degradation in vivo. Nutrient upshifts trigger RpoS degradation independently of protein synthesis by activating metabolic pathways that generate small energy molecules. Using metabolic mutants and inhibitors, we show that ATP, but not GTP or NADH, is necessary for RpoS degradation. In vitro reconstitution assays directly demonstrate that ClpXP fails to degrade RpoS, but not other proteins, at low ATP hydrolysis rates. These data suggest that cellular ATP levels directly control RpoS stability.
KW - Cellular energy
KW - Metabolic state
KW - Protein unfolding
KW - Stationary phase
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U2 - 10.1101/gad.183517.111
DO - 10.1101/gad.183517.111
M3 - Article
C2 - 22426532
AN - SCOPUS:84858310738
SN - 0890-9369
VL - 26
SP - 548
EP - 553
JO - Genes and Development
JF - Genes and Development
IS - 6
ER -