Rotational diffusion of cell surface components by time-resolved phosphorescence anisotropy.

R. H. Austin, S. S. Chan, T. M. Jovin

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

The rotational diffusion of concanavalin A receptors of viable Friend erythroleukemia cells and the band 3 anion transport system of human erythrocytes has been measured via the time-dependent phosphorescence emission intensity and anisotropy of triplet probes excited by a 5-ns laser pulse. High-quality phosphorescence decay curves with a 10-microseconds time resolution were obtained at concentrations of the eosin probe down to 20 nM and in aqueous media at temperatures of 4-38 degrees C. A strong temperature dependence in the rotational behavior was observed for the band 3 anion transport protein, but the lectin receptors of the Friend erythroleukemia cells were found to be immobile on the time scale of 1-4000 microseconds at either 4 degrees C or 37 degrees C. The technique is applicable to other triplet probes and membrane components of living cells under conditions that do not destroy viability.

Original languageEnglish (US)
Pages (from-to)5650-5654
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number11
DOIs
StatePublished - Nov 1979

All Science Journal Classification (ASJC) codes

  • General

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