Ribosomal route to small-molecule diversity

Ma Diarey B. Tianero; Mohamed S. Donia; Travis S. Young; Peter G. Schultz; Eric W. Schmidt

doi:10.1021/ja208278k

Ribosomal route to small-molecule diversity

Ma Diarey B. Tianero
, Mohamed S. Donia
, Travis S. Young
, Peter G. Schultz
, Eric W. Schmidt

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in Escherichia coli. These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.

Original language	English (US)
Pages (from-to)	418-425
Number of pages	8
Journal	Journal of the American Chemical Society
Volume	134
Issue number	1
DOIs	https://doi.org/10.1021/ja208278k
State	Published - Jan 11 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Chemistry
Biochemistry
Catalysis
Colloid and Surface Chemistry

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10.1021/ja208278k

Cite this

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title = "Ribosomal route to small-molecule diversity",

abstract = "The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in Escherichia coli. These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.",

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doi = "10.1021/ja208278k",

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AU - Tianero, Ma Diarey B.

AU - Donia, Mohamed S.

AU - Young, Travis S.

AU - Schultz, Peter G.

AU - Schmidt, Eric W.

PY - 2012/1/11

Y1 - 2012/1/11

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AB - The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in Escherichia coli. These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.

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UR - https://www.scopus.com/pages/publications/84855646831#tab=citedBy

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EP - 425

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JF - Journal of the American Chemical Society

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Ribosomal route to small-molecule diversity

Abstract

All Science Journal Classification (ASJC) codes

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