@article{38cdc52fe8144e0a86c52220ec479944,
title = "Rewilding Nod2 and Atg16l1 Mutant Mice Uncovers Genetic and Environmental Contributions to Microbial Responses and Immune Cell Composition",
abstract = "The effect of genetics verses environment on immunity is incompletely understood. By releasing laboratory mice carrying IBD susceptibility genes into the outdoors, Lin et al. find that exposure to environmental microbes promotes variation in immune cell populations, whereas cytokine responses to microbial stimulation are affected more by genetic IBD susceptibility.",
keywords = "immune variation, inflammatory bowel disease, machine learning model, multi-omic data integration, rewilded mice",
author = "Lin, {Jian Da} and Devlin, {Joseph C.} and Frank Yeung and Caroline McCauley and Leung, {Jacqueline M.} and Chen, {Ying Han} and Alex Cronkite and Christina Hansen and Charlotte Drake-Dunn and Ruggles, {Kelly V.} and Ken Cadwell and Graham, {Andrea L.} and P'ng Loke",
note = "Funding Information: K.C. receives research funding from Pfizer and Abbvie, and P.L. receives research funding from Pfizer. K.C. has consulted for or received an honorarium from Puretech Health, Genentech, and Abbvie. P.L. consults for and has equity in Toilabs. K.C. has a provisional patent, U.S. Patent Appln. No. 15/625,934. P.L. is a federal employee. All other authors declare no competing interests. Funding Information: We wish to thank William Craigens, Daniel Navarrete Prado, Allison Lee, and Veena Chittamuri for assistance with trapping and husbandry in the field and the PU Lab Animal Resources staff for logistical support. We wish to thank the NYU School of Medicine Flow Cytometry and Cell Sorting, Microscopy, Genome Technology, and Histology Cores for use of their instruments and technical assistance (supported in part by National Institute of Health [NIH] grants P31CA016087, S10OD01058, and S10OD018338). This research was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, NIH and also NIH grants DK103788 to K.C. and P.L.; AI121244, HL123340, and DK093668 to K.C.; AI130945 to P.L. and K.C.; R01 HL125816 and R01 AI140754 to K.C.; HL084312 and AI133977 to P.L.; research station and research rebate awards from Princeton University, Department of Ecology and Evolutionary Biology (PU EEB) to A.L.G.; a pilot award from the NYU CTSA grant UL1TR001445 from the National Center for Advancing Translational Sciences (NCATS) to K.C. and P.L.; a pilot award from the NYU Cancer Center grant P30CA016087 to K.C.; AI100853 to Y.C.; and DK122698 to F.Y. This work was also supported by the Department of Defense grant W81XWH-16-1-0256 to P.L.; a Faculty Scholar grant from the Howard Hughes Medical Institute, the Crohn's & Colitis Foundation, Merieux Institute, the Kenneth Rainin Foundation, and the Stony-Wold Herbert Fund to K.C. and a Bernard Levine Postdoctoral Research Fellowship in Immunology to Y.C. K.C. is a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Diseases. Design of experiments, data analysis, data discussion, and interpretation: J.D.L. J.C.D. F.Y. K.C. A.L.G, and P.L.; primary responsibility for execution of experiments: J.D.L. F.Y. C.M. J.M.L. Y.H.C. A.C. C.H. and C.D.D.; MLN cell RNA and 16S analysis: J.C.D. K.V.R. J.D.L. and F.Y. Supervised and Unsupervised machine learning model analysis: J.C.D. K.V.R. and J.D.L. All authors discussed data and commented on the manuscript. K.C. receives research funding from Pfizer and Abbvie, and P.L. receives research funding from Pfizer. K.C. has consulted for or received an honorarium from Puretech Health, Genentech, and Abbvie. P.L. consults for and has equity in Toilabs. K.C. has a provisional patent, U.S. Patent Appln. No. 15/625,934. P.L. is a federal employee. All other authors declare no competing interests. Funding Information: We wish to thank William Craigens, Daniel Navarrete Prado, Allison Lee, and Veena Chittamuri for assistance with trapping and husbandry in the field and the PU Lab Animal Resources staff for logistical support. We wish to thank the NYU School of Medicine Flow Cytometry and Cell Sorting, Microscopy, Genome Technology, and Histology Cores for use of their instruments and technical assistance (supported in part by National Institute of Health [NIH] grants P31CA016087 , S10OD01058 , and S10OD018338 ). This research was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases , NIH and also NIH grants DK103788 to K.C. and P.L.; AI121244 , HL123340 , and DK093668 to K.C.; AI130945 to P.L. and K.C.; R01 HL125816 and R01 AI140754 to K.C.; HL084312 and AI133977 to P.L.; research station and research rebate awards from Princeton University , Department of Ecology and Evolutionary Biology (PU EEB) to A.L.G.; a pilot award from the NYU CTSA grant UL1TR001445 from the National Center for Advancing Translational Sciences (NCATS) to K.C. and P.L.; a pilot award from the NYU Cancer Center grant P30CA016087 to K.C.; AI100853 to Y.C.; and DK122698 to F.Y. This work was also supported by the Department of Defense grant W81XWH-16-1-0256 to P.L.; a Faculty Scholar grant from the Howard Hughes Medical Institute , the Crohn{\textquoteright}s & Colitis Foundation , Merieux Institute , the Kenneth Rainin Foundation , and the Stony-Wold Herbert Fund to K.C., and a Bernard Levine Postdoctoral Research Fellowship in Immunology to Y.C. K.C. is a Burroughs Wellcome Fund Investigator in the Pathogenesis of Infectious Diseases. Publisher Copyright: {\textcopyright} 2020",
year = "2020",
month = may,
day = "13",
doi = "10.1016/j.chom.2020.03.001",
language = "English (US)",
volume = "27",
pages = "830--840.e4",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "5",
}