Restriction mapping in nanofluidic devices

Robert Riehn, Manchun Lu, Yan Mei Wang, Shuang Fang Lim, Edward C. Cox, Robert H. Austin

Research output: Contribution to journalArticle

187 Scopus citations

Abstract

We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of ≈1.5 kbp in 1 min using single DNA molecules.

Original languageEnglish (US)
Pages (from-to)10012-10016
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number29
DOIs
StatePublished - Jul 19 2005

All Science Journal Classification (ASJC) codes

  • General

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