Restriction mapping in nanofluidic devices

Robert Riehn; Manchun Lu; Yan Mei Wang; Shuang Fang Lim; Edward C. Cox; Robert H. Austin

doi:10.1073/pnas.0503809102

Restriction mapping in nanofluidic devices

Robert Riehn, Manchun Lu, Yan Mei Wang, Shuang Fang Lim, Edward C. Cox, Robert H. Austin

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199 Scopus citations

Abstract

We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg²⁺ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of ≈1.5 kbp in 1 min using single DNA molecules.

Original language	English (US)
Pages (from-to)	10012-10016
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	29
DOIs	https://doi.org/10.1073/pnas.0503809102
State	Published - Jul 19 2005

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abstract = "We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of ≈1.5 kbp in 1 min using single DNA molecules.",

author = "Robert Riehn and Manchun Lu and Wang, {Yan Mei} and Lim, {Shuang Fang} and Cox, {Edward C.} and Austin, {Robert H.}",

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T1 - Restriction mapping in nanofluidic devices

AU - Riehn, Robert

AU - Lu, Manchun

AU - Wang, Yan Mei

AU - Lim, Shuang Fang

AU - Cox, Edward C.

AU - Austin, Robert H.

PY - 2005/7/19

Y1 - 2005/7/19

N2 - We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of ≈1.5 kbp in 1 min using single DNA molecules.

AB - We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites with a precision of ≈1.5 kbp in 1 min using single DNA molecules.

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U2 - 10.1073/pnas.0503809102

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 29

ER -

Restriction mapping in nanofluidic devices

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