Response regulator output in bacterial chemotaxis

Uri Alon, Laura Camarena, Michael G. Surette, Blaise Aguera Y Arcas, Yi Liu, Stanislas Leibler, Jeffry B. Stock

Research output: Contribution to journalArticle

162 Scopus citations

Abstract

Chemotaxis responses in Escherichia coli are mediated by the phosphorylated response-regulator protein P-CheY. Biochemical and genetic studies have established the mechanisms by which the various components of the chemotaxis system, the membrane receptors and Che proteins function to modulate levels of CheY phosphorylation. Detailed models have been formulated to explain chemotaxis sensing in quantitative terms; however, the models cannot be adequately tested without knowledge of the quantitative relationship between P-CheY and bacterial swimming behavior. A computerized image analysis system was developed to collect extensive statistics on free-swimming and individual tethered cells. P-CheY levels were systematically varied by controlled expression of CheY in an E.coli strain lacking the CheY phosphatase, CheZ, and the receptor demethylating enzyme CheB. Tumbling frequency was found to vary with P-CheY concentration in a weakly sigmoidal fashion (apparent Hill coefficient ~ 2.5). This indicates that the high sensitivity of the chemotaxis system is not derived from highly cooperative interactions between P-CheY and the flagellar motor, but rather depends on nonlinear effects within the chemotaxis signal transduction network. The complex relationship between single flagella rotation and free-swimming behavior was examined; our results indicate that there is an additional level of information processing associated with interactions between the individual flagella. An allosteric model of the motor switching process is proposed which gives a good fit to the observed switching induced by P-CheY. Thus the level of intracellular P-CheY can be estimated from behavior determinations: ~ 30% of the intracellular pool of CheY appears to be phosphorylated in fully adapted wild-type cells.

Original languageEnglish (US)
Pages (from-to)4238-4248
Number of pages11
JournalEMBO Journal
Volume17
Issue number15
DOIs
StatePublished - Aug 3 1998

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Keywords

  • Bacterial swimming
  • Escherichia coli
  • Image analysis
  • P-CheY

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