Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts

W. A. Bresnahan, G. E. Hultman, T. Shenk

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

Original languageEnglish (US)
Pages (from-to)10816-10818
Number of pages3
JournalJournal of virology
Volume74
Issue number22
DOIs
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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