Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts

W. A. Bresnahan; G. E. Hultman; T. Shenk

doi:10.1128/JVI.74.22.10816-10818.2000

Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts

W. A. Bresnahan, G. E. Hultman, T. Shenk

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102 Scopus citations

Abstract

A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

Original language	English (US)
Pages (from-to)	10816-10818
Number of pages	3
Journal	Journal of virology
Volume	74
Issue number	22
DOIs	https://doi.org/10.1128/JVI.74.22.10816-10818.2000
State	Published - 2000

All Science Journal Classification (ASJC) codes

Insect Science
Virology
Microbiology
Immunology

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10.1128/JVI.74.22.10816-10818.2000

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abstract = "A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.",

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AB - A cDNA encoding the catalytic subunit of human telomerase was used to generate life-extended derivatives of primary human diploid fibroblasts. The life-extended cells supported efficient human cytomegalovirus (HCMV) replication. A subclone of the life-extended cells was generated containing the HCMV UL82 gene and used to isolate and propagate a virus that exhibited a profound growth defect after infection at a low input multiplicity.

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Replication of wild-type and mutant human cytomegalovirus in life-extended human diploid fibroblasts

Abstract

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