Removal of drosophila muscle tissue from larval fillets for immunofluorescence analysis of sensory neurons and epidermal cells

Conrad M. Tenenbaum, Elizabeth R. Gavis

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Drosophila larval dendritic arborization (da) neurons are a popular model for investigating mechanisms of neuronal morphogenesis. Da neurons develop in communication with the epidermal cells they innervate and thus their analysis benefits from in situ visualization of both neuronally and epidermally expressed proteins by immunofluorescence. Traditional methods of preparing larval fillets for immunofluorescence experiments leave intact the muscle tissue that covers most of the body wall, presenting several challenges to imaging neuronal and epidermal proteins. Here we describe a method for removing muscle tissue from Drosophila larval fillets. This protocol enables imaging of proteins that are otherwise obscured by muscle tissue, improves signal to noise ratio, and facilitates the use of super-resolution microscopy to study da neuron development.

Original languageEnglish (US)
Article numbere54670
JournalJournal of Visualized Experiments
Volume2016
Issue number117
DOIs
StatePublished - Nov 2 2016

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Keywords

  • Dendritic arborization neuron
  • Dissection
  • Drosophila
  • Epidermis
  • Fillet
  • Issue 117
  • Larva
  • Muscle
  • Neuroscience

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