Remodeling of the metabolome during early frog development

Livia Vastag, Paul Jorgensen, Leonid Peshkin, Ru Wei, Joshua D. Rabinowitz, Marc W. Kirschner

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

A rapid series of synchronous cell divisions initiates embryogenesis in many animal species, including the frog Xenopus laevis. After many of these cleavage cycles, the nuclear to cytoplasmic ratio increases sufficiently to somehow cause cell cycles to elongate and become asynchronous at the mid-blastula transition (MBT). We have discovered that an unanticipated remodeling of core metabolic pathways occurs during the cleavage cycles and the MBT in X.laevis, as evidenced by widespread changes in metabolite abundance. While many of the changes in metabolite abundance were consistently observed, it was also evident that different female frogs laid eggs with different levels of at least some metabolites. Metabolite tracing with heavy isotopes demonstrated that alanine is consumed to generate energy for the early embryo. dATP pools were found to decline during the MBT and we have confirmed that maternal pools of dNTPs are functionally exhausted at the onset of the MBT. Our results support an alternative hypothesis that the cell cycle lengthening at the MBT is triggered not by a limiting maternal protein, as is usually proposed, but by a decline in dNTP pools brought about by the exponentially increasing demands of DNA synthesis.

Original languageEnglish (US)
Article numbere16881
JournalPloS one
Volume6
Issue number2
DOIs
StatePublished - 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Remodeling of the metabolome during early frog development'. Together they form a unique fingerprint.

  • Cite this

    Vastag, L., Jorgensen, P., Peshkin, L., Wei, R., Rabinowitz, J. D., & Kirschner, M. W. (2011). Remodeling of the metabolome during early frog development. PloS one, 6(2), [e16881]. https://doi.org/10.1371/journal.pone.0016881