@article{bab07417570d453a92a836b0c8adba12,
title = "Recurrent SMARCB1 Mutations Reveal a Nucleosome Acidic Patch Interaction Site That Potentiates mSWI/SNF Complex Chromatin Remodeling",
abstract = "The SMARCB1 C-terminal alpha-helical domain binds the nucleosome acidic patch and mediates mSWI/SNF chromatin remodeling.",
keywords = "ATP-dependent chromatin remodeling, BAF complex, Coffin-Siris syndrome, SMARCB1 (BAF47), chromatin accessibility, intellectual disability, mammalian SWI/SNF complexes, nucleosome acidic patch, nucleosome remodeling, structure",
author = "Valencia, {Alfredo M.} and Collings, {Clayton K.} and Dao, {Hai T.} and {St. Pierre}, Roodolph and Cheng, {Yung Chih} and Junwei Huang and Sun, {Zhen Yu} and Seo, {Hyuk Soo} and Nazar Mashtalir and Comstock, {Dawn E.} and Olubusayo Bolonduro and Vangos, {Nicholas E.} and Yeoh, {Zoe C.} and Dornon, {Mary Kate} and Crystal Hermawan and Lee Barrett and Sirano Dhe-Paganon and Woolf, {Clifford J.} and Muir, {Tom W.} and Cigall Kadoch",
note = "Funding Information: We thank members of the Kadoch lab for critical feedback and advice, as well as R.T. Nakayama and R.T. Williams for early technical assistance and conceptual guidance. We thank members of the A.M.V. dissertation advisory committee, Drs. Robert E. Kingston, David R. Liu, and Stephen J. Haggarty, for their guidance and mentorship throughout the development of this project. We thank M. Sahin for their original generation of the SAH (SAH0047-02) cell line. We also thank Yufan Yang for helping with REAA and ATPase experiments and Kyle Korshavn and Noah Bloch (L.D. Walensky laboratory) for helping to perform CD experiments. A.M.V. is supported by the Howard Hughes Medical Institute Gilliam Fellowship Program, the NIH (5 T32 GM095450-04), and the Ford Foundation Fellowship. This work was supported in part by awards from the NIH DP2 New Innovator Award (1DP2CA195762-01), the American Cancer Society Research Scholar Award (RSG-14-051-01-DMC), and the Pew-Stewart Scholars in Cancer Research Grant to C.K. C.J.W. was supported by NIH grants R35NS105076 and U54HD090255. T.W.M. was supported by NIH grants 5R37 GM086868 and P01 CA196539. A.M.V. and C.K. conceived of and designed the study. A.M.V, H.T.D. R.S. Y.C, J.H. Z.S. H.S. N.M. D.E.C. O.B. N.E.V. Z.C.Y. C.H. M.K.D. and L.B.B. performed experiments. C.K.C. performed all data analyses and statistical calculations in the paper. R.S. developed the REAA and ATPase assay experiments used in this study. S.D. supervised protein production for NMR experments. C.J.W. supervised neuronal differentiation experiments. T.W.M. supervised generation of recombinant nucleosomes and photocrosslinking experiments. C.K. supervised the study. A.M.V and C.K. wrote the paper. C.K. is a scientific founder, fiduciary Board of Directors member, Scientific Advisory Board member, consultant, and shareholder of Foghorn Therapeutics, Inc. (Cambridge, MA). C.J.W. is a founder of Nocion Therapeutics, Ferrumax, and QurAlis. The other authors declare no competing interests. Funding Information: We thank members of the Kadoch lab for critical feedback and advice, as well as R.T. Nakayama and R.T. Williams for early technical assistance and conceptual guidance. We thank members of the A.M.V. dissertation advisory committee, Drs. Robert E. Kingston, David R. Liu, and Stephen J. Haggarty, for their guidance and mentorship throughout the development of this project. We thank M. Sahin for their original generation of the SAH (SAH0047-02) cell line. We also thank Yufan Yang for helping with REAA and ATPase experiments and Kyle Korshavn and Noah Bloch (L.D. Walensky laboratory) for helping to perform CD experiments. A.M.V. is supported by the Howard Hughes Medical Institute Gilliam Fellowship Program , the NIH ( 5 T32 GM095450-04 ), and the Ford Foundation Fellowship . This work was supported in part by awards from the NIH DP2 New Innovator Award ( 1DP2CA195762-01 ), the American Cancer Society Research Scholar Award ( RSG-14-051-01-DMC ), and the Pew-Stewart Scholars in Cancer Research Grant to C.K. C.J.W. was supported by NIH grants R35NS105076 and U54HD090255 . T.W.M. was supported by NIH grants 5R37 GM086868 and P01 CA196539 . Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = nov,
day = "27",
doi = "10.1016/j.cell.2019.10.044",
language = "English (US)",
volume = "179",
pages = "1342--1356.e23",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}