TY - JOUR
T1 - Reconstructing the engram
T2 - Simultaneous, multisite, many single neuron recordings
AU - Nicolelis, Miguel A.L.
AU - Ghazanfar, Asif A.
AU - Faggin, Barbara M.
AU - Votaw, Scott
AU - Oliveira, Laura M.O.
PY - 1997/4
Y1 - 1997/4
N2 - Little is known about the physiological principles that govern large- scale neuronal interactions in the mammalian brain. Here, we describe an electrophysiological paradigm capable of simultaneously recording the extracellular activity of large populations of single neurons, distributed across multiple cortical and subcortical structures in behaving and anesthetized animals. Up to 100 neurons were simultaneously recorded after 48 microwires were implanted in the brain stem, thalamus, and somatosensory cortex of rats. Overall, 86% of the implanted microwires yielded single neurons, and an average of 2.3 neurons were discriminated per microwire. Our population recordings remained stable for weeks, demonstrating that this method can be employed to investigate the dynamic and distributed neuronal ensemble interactions that underlie processes such as sensory perception, motor control, and sensorimotor learning in freely behaving animals.
AB - Little is known about the physiological principles that govern large- scale neuronal interactions in the mammalian brain. Here, we describe an electrophysiological paradigm capable of simultaneously recording the extracellular activity of large populations of single neurons, distributed across multiple cortical and subcortical structures in behaving and anesthetized animals. Up to 100 neurons were simultaneously recorded after 48 microwires were implanted in the brain stem, thalamus, and somatosensory cortex of rats. Overall, 86% of the implanted microwires yielded single neurons, and an average of 2.3 neurons were discriminated per microwire. Our population recordings remained stable for weeks, demonstrating that this method can be employed to investigate the dynamic and distributed neuronal ensemble interactions that underlie processes such as sensory perception, motor control, and sensorimotor learning in freely behaving animals.
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U2 - 10.1016/S0896-6273(00)80295-0
DO - 10.1016/S0896-6273(00)80295-0
M3 - Article
C2 - 9136763
AN - SCOPUS:0030895755
VL - 18
SP - 529
EP - 537
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 4
ER -