Recent advances in the application of expressed protein ligation to protein engineering

Roseanne M. Hofmann; Tom W. Muir

doi:10.1016/S0958-1669(02)00326-9

Recent advances in the application of expressed protein ligation to protein engineering

Roseanne M. Hofmann
, Tom W. Muir

Research output: Contribution to journal › Review article › peer-review

82 Scopus citations

Abstract

Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.

Original language	English (US)
Pages (from-to)	297-303
Number of pages	7
Journal	Current Opinion in Biotechnology
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/S0958-1669(02)00326-9
State	Published - Aug 1 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Bioengineering
Biotechnology
Biomedical Engineering

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10.1016/S0958-1669(02)00326-9

Cite this

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title = "Recent advances in the application of expressed protein ligation to protein engineering",

abstract = "Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.",

author = "Hofmann, \{Roseanne M.\} and Muir, \{Tom W.\}",

note = "Funding Information: We thank members of the Muir laboratory for helpful comments on the manuscript. This work was supported by the National Institutes of Health and The Rockefeller University. ",

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AU - Hofmann, Roseanne M.

AU - Muir, Tom W.

N1 - Funding Information: We thank members of the Muir laboratory for helpful comments on the manuscript. This work was supported by the National Institutes of Health and The Rockefeller University.

PY - 2002/8/1

Y1 - 2002/8/1

N2 - Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.

AB - Expressed protein ligation is a technique for joining recombinantly expressed proteins to polypeptides containing biophysical probes, post-translational modifications or unnatural amino acids. Recent advances have expanded the scope of expressed protein ligation and have allowed the approach to be applied to the study of basic biological questions.

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UR - https://www.scopus.com/pages/publications/0036669566#tab=citedBy

U2 - 10.1016/S0958-1669(02)00326-9

DO - 10.1016/S0958-1669(02)00326-9

M3 - Review article

C2 - 12323349

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JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

IS - 4

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Recent advances in the application of expressed protein ligation to protein engineering

Abstract

All Science Journal Classification (ASJC) codes

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