@article{2eaef931d303473e993317b7c6fd2cbe,
title = "Recapitulation of the hepatitis C virus life-cycle in engineered murine cell lines",
abstract = "Hepatitis C virus (HCV) remains a major medical problem. In-depth study of HCV pathogenesis and immune responses is hampered by the lack of suitable small animal models. The narrow host range of HCV remains incompletely understood. We demonstrate that the entire HCV life-cycle can be recapitulated in mouse cells. We show that antiviral signaling interferes with HCV RNA replication in mouse cells. We were able to infect mouse cells expressing human CD81 and occludin (OCLN)-the minimal set of entry factor factors required for HCV uptake into mouse cells. Infected mouse cells sustain HCV RNA replication in the presence of miR122 and release infectious particles when mouse apoE is supplied. Our data demonstrate that the barriers of HCV interspecies transmission can be overcome by engineering a suitable cellular environment and provide a blue-print towards constructing a small animal model for HCV infection.",
keywords = "HCV animal model, Hepatitis C virus, Innate immunity, Species tropism, Viral assembly, Viral entry",
author = "Alexander Vogt and Scull, {Margaret A.} and Tamar Friling and Horwitz, {Joshua A.} and Donovan, {Bridget M.} and Marcus Dorner and Gisa Gerold and Labitt, {Rachael N.} and Rice, {Charles M.} and Alexander Ploss",
note = "Funding Information: This study was supported in part by Award no. RC1DK087193 (to C.M.R. and A.P.) from the National Institute of Diabetes and Digestive and Kidney Diseases , R01AI072613 (to C.M.R.) from the National Institute for Allergy and Infectious Disease , The Starr Foundation and the Greenberg Medical Institute. A.V. was supported by a stipend from the International Institute of Life Sciences (IALS), M.A.S by a Ruth L. Kirschstein NRSA postdoctoral fellowship (Award no. F32AI091207 ) from the National Institute Of Allergy And Infectious Diseases, M.D. by postdoctoral fellowship from the German Research Foundation (Deutsche Forschungsgesellschaft) and G.G. was supported by the German Academy of Sciences Leopoldina ( LPDS2009-9 ) and by the Human FrontierScience Program ( LT000048/2009 ). A.P. is a recipient of Astella Young Investigator Award from the Infectious Disease Society of America and a Liver Scholar Award from the American Liver Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.",
year = "2013",
month = sep,
doi = "10.1016/j.virol.2013.05.036",
language = "English (US)",
volume = "444",
pages = "1--11",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1-2",
}