@article{114952570d374228aad172cc08e1e798,
title = "Real-time imaging of hepatitis C virus infection using a fluorescent cell-based reporter system",
abstract = "Hepatitis C virus (HCV), which infects 2-3% of the world population, is a causative agent of chronic hepatitis and the leading indication for liver transplantation. The ability to propagate HCV in cell culture (HCVcc) is a relatively recent breakthrough and a key tool in the quest for specific antiviral therapeutics. Monitoring HCV infection in culture generally involves bulk population assays, use of genetically modified viruses and/or terminal processing of potentially precious samples. Here we develop a cell-based fluorescent reporter system that allows sensitive distinction of individual HCV-infected cells in live or fixed samples. We demonstrate use of this technology for several previously intractable applications, including live-cell imaging of viral propagation and host response, as well as visualizing infection of primary hepatocyte cultures. Integration of this reporter with modern image-based analysis methods could open new doors for HCV research.",
author = "Jones, {Christopher T.} and Catanese, {Maria Teresa} and Law, {Lok Man J.} and Khetani, {Salman R.} and Syder, {Andrew J.} and Alexander Ploss and Oh, {Thomas S.} and Schoggins, {John W.} and MacDonald, {Margaret R.} and Bhatia, {Sangeeta N.} and Rice, {Charles M.}",
note = "Funding Information: We acknowledge the expert support of The Rockefeller University Bioimaging Core Facility, with special thanks to A. North, S. Galdeen and S. Bhuvanendran. We thank The Rockefeller University Flow Cytometry Resource Center, supported by the Empire State Stem Cell Fund through NY State Department of Health (NYSDOH) contract no. C023046; opinions expressed here are solely those of the authors and do not necessarily reflect those of the Empire State Stem Cell Fund, the NYSDOH, or the State of NY. We are grateful to C. Stoyanov (The Rockefeller University) for YF17D(5′C25Venus2AUbi), J. Tazi for G3BP (Institut de G{\'e}n{\'e}tique Mol{\'e}culaire de Montpellier) and I. Frolov (UTMB) for Venezuelan equine encephalitis virus-EGFP. We thank M. Holz, A. Forest, M. Panis and A. Webson for laboratory support and C. Murray for critical reading of the manuscript. This work was supported by Public Health Service grants R01 AI075099 (C.M.R.) and R01 DK56966 (S.N.B.). This work was also funded by the Office of the Director/National Institutes of Health (NIH) through the NIH Roadmap for Medical Research, Grant 1 R01 DK085713-01 (C.M.R. and S.N.B.). Information on this Roadmap Transformative R01 Program can be found at http://grants.nih. gov/grants/guide/rfa-files/RFA-RM-08-029.html. Additional funding was provided by the Greenberg Medical Research Institute and the Starr Foundation (C.M.R.). S.N.B. is an Howard Hughes Medical Investigator investigator. C.T.J. was supported by National Research Service Award DK081193; M.T.C. was supported by a Women & Science Fellowship; L.M.J.L. is supported by a Natural Sciences and Engineering Research Council of Canada fellowship. A.P. is a recipient of a Kimberly Lawrence-Netter cancer research discovery fund award.",
year = "2010",
month = feb,
doi = "10.1038/nbt.1604",
language = "English (US)",
volume = "28",
pages = "167--171",
journal = "Nature biotechnology",
issn = "1087-0156",
publisher = "Nature Research",
number = "2",
}