Real Time Cytokine Quantification in Wound Fluid Samples Using Nanowell Impedance Sensing

Pengfei Xie, Muhammad Tayyab, Ali Ashraf, Suneel Kumar, Aaron Mazzeo, Kaushik Sengupta, Francois Berthiaume, Mehdi Javanmard

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cytokine levels in skin wound fluid can provide valuable information on the progression of the local healing phases and help in making therapeutic decisions. The gold standard cytokine quantification method is the enzyme-linked immunosorbent assay (ELISA), which typically relies on labeling and an optical read-out. Impedance sensors provide a promising alternative whose advantages include ease of miniaturization, rapid readout, and label-free operation. We present a nanowell impedance sensor for label free cytokine quantification in wound fluid samples. The sensor relies on the nanowell array structure to perform the label free cytokine detection with high sensitivity despite the high salt concentration of wound fluid. Real time cytokine detection is achieved in the experiments by continuous monitoring of the impedance of the sensor using lock-in amplifier, which increases due to the target protein binding. Multiplexing measurements are also achieved using a multiplexer chip in conjunction with an embedded microprocessor.

Original languageEnglish (US)
Title of host publication21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages779-782
Number of pages4
ISBN (Electronic)9781665412674
DOIs
StatePublished - Jun 20 2021
Event21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021 - Virtual, Online, United States
Duration: Jun 20 2021Jun 25 2021

Publication series

Name21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021

Conference

Conference21st International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2021
Country/TerritoryUnited States
CityVirtual, Online
Period6/20/216/25/21

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Control and Optimization
  • Instrumentation

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